Molecular cloning and stress-dependent regulation of a glutamine synthetase gene in Chinese cabbage

Molecular cloning and stress-dependent regulation of a glutamine synthetase gene in Chinese cabbage A new glutamine synthetase (GS, EC 6.3.1.2) gene C-GS1 (GenBank accession no.AY773089) was isolated from Chinese cabbage (Brassica rapa ssp. pekinensis). Sequence and structural analysis showed that C-GS1 protein contained structural domains conserved within plant GS1s. The amino acid sequence of C-GS1 had extensive homology with those of GS1s from other higher plants. The phylogenetic tree displayed that the C-GS1 was the closest to GS1 from Brassica napus. C-GS1 was preferentially expressed in roots and stems rather than in leaves. Cold treatment (4°C) and water logging stress enhanced and abscisic acid, H2O2, and salicylic acid repressed the levels of C-GS1 transcripts. This study indicates that stress factors regulate the C-GS1 expression at the transcriptional level. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Molecular cloning and stress-dependent regulation of a glutamine synthetase gene in Chinese cabbage

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Publisher
Nauka/Interperiodica
Copyright
Copyright © 2006 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443706020130
Publisher site
See Article on Publisher Site

Abstract

A new glutamine synthetase (GS, EC 6.3.1.2) gene C-GS1 (GenBank accession no.AY773089) was isolated from Chinese cabbage (Brassica rapa ssp. pekinensis). Sequence and structural analysis showed that C-GS1 protein contained structural domains conserved within plant GS1s. The amino acid sequence of C-GS1 had extensive homology with those of GS1s from other higher plants. The phylogenetic tree displayed that the C-GS1 was the closest to GS1 from Brassica napus. C-GS1 was preferentially expressed in roots and stems rather than in leaves. Cold treatment (4°C) and water logging stress enhanced and abscisic acid, H2O2, and salicylic acid repressed the levels of C-GS1 transcripts. This study indicates that stress factors regulate the C-GS1 expression at the transcriptional level.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Mar 24, 2006

References

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