A fragment of the gene des3-1 encoding ω3 fatty acid desaturase was cloned from a cDNA library of the unicellular green galophilic alga Dunaliella salina. The comparative phylogenetic analysis of ω3-desaturase amino acid sequences from diverse organisms placed the desaturase of D. salina between cyanobacteria and higher plants in the evolutionary range of desaturases. The expression of des3-1 was studied in D. salina cells exposed to low temperatures, high irradiance, and high CO2 concentrations. Lowering the external temperature from 32 to 22°C produced a transient increase in the level of specific mRNA. Considerable accumulation of mRNA for ω3-desaturase was also observed when CO2 concentration in gas–air mixture was raised from 2 to 10%. An irradiation increase from 70 to 500 μmol/(m2 s) did not affect the level of specific mRNA. The latter evidence presumes that in Dunaliella cells, this desaturase is probably located in the endoplasmic reticulum, rather than in the chloroplast.
Russian Journal of Plant Physiology – Springer Journals
Published: Oct 11, 2004
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