Molecular Cloning and Stress-Dependent Expression of a Gene Encoding ω3-Fatty Acid Desaturase in the Microalga Dunaliella salina

Molecular Cloning and Stress-Dependent Expression of a Gene Encoding ω3-Fatty Acid Desaturase in... A fragment of the gene des3-1 encoding ω3 fatty acid desaturase was cloned from a cDNA library of the unicellular green galophilic alga Dunaliella salina. The comparative phylogenetic analysis of ω3-desaturase amino acid sequences from diverse organisms placed the desaturase of D. salina between cyanobacteria and higher plants in the evolutionary range of desaturases. The expression of des3-1 was studied in D. salina cells exposed to low temperatures, high irradiance, and high CO2 concentrations. Lowering the external temperature from 32 to 22°C produced a transient increase in the level of specific mRNA. Considerable accumulation of mRNA for ω3-desaturase was also observed when CO2 concentration in gas–air mixture was raised from 2 to 10%. An irradiation increase from 70 to 500 μmol/(m2 s) did not affect the level of specific mRNA. The latter evidence presumes that in Dunaliella cells, this desaturase is probably located in the endoplasmic reticulum, rather than in the chloroplast. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Molecular Cloning and Stress-Dependent Expression of a Gene Encoding ω3-Fatty Acid Desaturase in the Microalga Dunaliella salina

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Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2003 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1023/A:1024764522062
Publisher site
See Article on Publisher Site

Abstract

A fragment of the gene des3-1 encoding ω3 fatty acid desaturase was cloned from a cDNA library of the unicellular green galophilic alga Dunaliella salina. The comparative phylogenetic analysis of ω3-desaturase amino acid sequences from diverse organisms placed the desaturase of D. salina between cyanobacteria and higher plants in the evolutionary range of desaturases. The expression of des3-1 was studied in D. salina cells exposed to low temperatures, high irradiance, and high CO2 concentrations. Lowering the external temperature from 32 to 22°C produced a transient increase in the level of specific mRNA. Considerable accumulation of mRNA for ω3-desaturase was also observed when CO2 concentration in gas–air mixture was raised from 2 to 10%. An irradiation increase from 70 to 500 μmol/(m2 s) did not affect the level of specific mRNA. The latter evidence presumes that in Dunaliella cells, this desaturase is probably located in the endoplasmic reticulum, rather than in the chloroplast.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 11, 2004

References

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