Molecular cloning and mRNA localization of tomato pollen profilin

Molecular cloning and mRNA localization of tomato pollen profilin The actin cytoskeleton plays an important role in the growth of pollen tube. The actin-binding protein profilin could play a role in regulating the organization of the actin filaments. Using the RT-PCR technique, we isolated a cDNA clone (designated LePro1) encoding profilin from pollen grains of tomato (Lycopersicon esculentum Mill. cv. Moneymaker). Sequence analysis of the insert shows 87% similarity to tobacco ntPro2, 78% to timothy grass profilin, 77% to Arabidopsis AthPRF4, 77% to maize ZmPro3, and 73% to birch profilin. Both quantitative PCR and RNA gel blot analyses demonstrated that LePro1 is expressed in a tissue- or cell-type specific manner in the tomato plant. In situ hybridization of 2 µm thick anther sections using a non-radioactive labeling method reveals that LePro1 is expressed only in pollen grains, with undetectable transcription in other parts of the anther or other organs. Phylogenetic analysis of amino acid sequences of 18 plant profilins indicates that two distinct profilin gene classes are present in higher plants. One is pollen-specific, another is constitutive. LePro1 belongs to the former class. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Molecular cloning and mRNA localization of tomato pollen profilin

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Publisher
Kluwer Academic Publishers
Copyright
Copyright © 1998 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1005971327353
Publisher site
See Article on Publisher Site

Abstract

The actin cytoskeleton plays an important role in the growth of pollen tube. The actin-binding protein profilin could play a role in regulating the organization of the actin filaments. Using the RT-PCR technique, we isolated a cDNA clone (designated LePro1) encoding profilin from pollen grains of tomato (Lycopersicon esculentum Mill. cv. Moneymaker). Sequence analysis of the insert shows 87% similarity to tobacco ntPro2, 78% to timothy grass profilin, 77% to Arabidopsis AthPRF4, 77% to maize ZmPro3, and 73% to birch profilin. Both quantitative PCR and RNA gel blot analyses demonstrated that LePro1 is expressed in a tissue- or cell-type specific manner in the tomato plant. In situ hybridization of 2 µm thick anther sections using a non-radioactive labeling method reveals that LePro1 is expressed only in pollen grains, with undetectable transcription in other parts of the anther or other organs. Phylogenetic analysis of amino acid sequences of 18 plant profilins indicates that two distinct profilin gene classes are present in higher plants. One is pollen-specific, another is constitutive. LePro1 belongs to the former class.

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 6, 2004

References

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