Molecular cloning and expression of two plasma membrane protein 3 (SmPMP3) genes from Salvia miltiorrhiza

Molecular cloning and expression of two plasma membrane protein 3 (SmPMP3) genes from Salvia... To ensure survival, plants must adjust to various abiotic stresses in their environment. Identifying their stress-responsive genes is important for understanding the molecular mechanisms underlying the process of adaptation. From Salvia miltiorrhiza Bunge seedlings, we isolated and characterized two stress-related genes that are highly conserved. Both plasma membrane protein 3 genes (SmPMP3-1 and SmPMP3-2) encode two low-molecular-weight hydrophobic proteins. Cloning of their 5′-flanking regions revealed that they contain many stress-related cis-acting regulatory elements and putative protein-binding sites. Although expression of both was induced by salt and abscisic acid, treatment with salicylate, methyl jasmonate, cold, and dehydration led to down-regulation of SmPMP3-1, whereas SmPMP3-2 was up-regulation. Thus, our results demonstrated the important regulatory effects of SmPMP3 in response to various abiotic stresses. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Molecular cloning and expression of two plasma membrane protein 3 (SmPMP3) genes from Salvia miltiorrhiza

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2013 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443712060179
Publisher site
See Article on Publisher Site

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