Molecular cloning and expression of two plasma membrane protein 3 (SmPMP3) genes from Salvia miltiorrhiza

Molecular cloning and expression of two plasma membrane protein 3 (SmPMP3) genes from Salvia... To ensure survival, plants must adjust to various abiotic stresses in their environment. Identifying their stress-responsive genes is important for understanding the molecular mechanisms underlying the process of adaptation. From Salvia miltiorrhiza Bunge seedlings, we isolated and characterized two stress-related genes that are highly conserved. Both plasma membrane protein 3 genes (SmPMP3-1 and SmPMP3-2) encode two low-molecular-weight hydrophobic proteins. Cloning of their 5′-flanking regions revealed that they contain many stress-related cis-acting regulatory elements and putative protein-binding sites. Although expression of both was induced by salt and abscisic acid, treatment with salicylate, methyl jasmonate, cold, and dehydration led to down-regulation of SmPMP3-1, whereas SmPMP3-2 was up-regulation. Thus, our results demonstrated the important regulatory effects of SmPMP3 in response to various abiotic stresses. Russian Journal of Plant Physiology Springer Journals

Molecular cloning and expression of two plasma membrane protein 3 (SmPMP3) genes from Salvia miltiorrhiza

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SP MAIK Nauka/Interperiodica
Copyright © 2013 by Pleiades Publishing, Ltd.
Life Sciences; Plant Physiology; Plant Sciences
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