Molecular cloning and expression characterization of a rice K+ channel β subunit

Molecular cloning and expression characterization of a rice K+ channel β subunit K+ channel proteins native to animal membranes have been shown to be composed of two different types of polypeptides: the pore-forming α subunit and the β subunit which may be involved in either modulation of conductance through the channel, or stabilization and surface expression of the channel complex. Several cDNAs encoding animal K+ channel β subunits have been recently cloned and sequenced. We report the molecular cloning of a rice plant homolog of these animal β subunits. The rice cDNA (KOB1) described in this report encodes a 36 kDa polypeptide which shares 45% sequence identity with these animal K+ channel β subunits, and 72% identity with the only other cloned plant (Arabidopsis thaliana) K+ channel β subunit (KAB1). The KOB1 translation product was demonstrated to form a tight physical association with a plant K+ channel α subunit. These results are consistent with the conclusion that the KOB1 cDNA encodes a K+ channel β subunit. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Molecular cloning and expression characterization of a rice K+ channel β subunit

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Publisher
Kluwer Academic Publishers
Copyright
Copyright © 1998 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1005913629485
Publisher site
See Article on Publisher Site

Abstract

K+ channel proteins native to animal membranes have been shown to be composed of two different types of polypeptides: the pore-forming α subunit and the β subunit which may be involved in either modulation of conductance through the channel, or stabilization and surface expression of the channel complex. Several cDNAs encoding animal K+ channel β subunits have been recently cloned and sequenced. We report the molecular cloning of a rice plant homolog of these animal β subunits. The rice cDNA (KOB1) described in this report encodes a 36 kDa polypeptide which shares 45% sequence identity with these animal K+ channel β subunits, and 72% identity with the only other cloned plant (Arabidopsis thaliana) K+ channel β subunit (KAB1). The KOB1 translation product was demonstrated to form a tight physical association with a plant K+ channel α subunit. These results are consistent with the conclusion that the KOB1 cDNA encodes a K+ channel β subunit.

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 6, 2004

References

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