Molecular cloning and characterization of the Strictosidine synthase Gene from Rauwolfia verticillata

Molecular cloning and characterization of the Strictosidine synthase Gene from Rauwolfia... The strictosidine synthase (STR, EC 4.3.3.2) catalyzes the condensation of tryptamine and secologanin to form strictosidine, which is the universal precursor for a wide range of pharmaceutical terpenoid indole alkaloids (TIAs). The full-length cDNA encoding STR was cloned and characterized from Rauwolfia verticillata a Chinese native plant producing TIAs, such as reserpine and ajmalicine. The new cDNA was designed as RvSTR and submitted to GenBank to get an accession number DQ017054. The full-length cDNA of RvSTR was 1211 bp containing a 1035-bp open reading frame encoding a deduced 344-amino-acid polypeptide with a calculated mol wt of 38.2 kD and an isoelectric point of 5.19. Comparative and bioinformatic analysis revealed that RvSTR showed a higher similarity to STRs from Apocynaceae species, including Catharanthus roseus and Ophiorrhiza pumila, but a relatively lower similarity to other plant STRs. The unique essential catalytic residue Glu-309 was conserved in all alignment plant species. The phylogenetic analysis revealed that STRs were divided into two groups, including plant and bacterial enzymes. The tissue expression pattern analysis indicated that RvSTR expression could be detected in all tested organs of R. verticillata, including roots, stems, leaves, fruits, and flowers. The lowest transcription level was observed in flowers and the highest was found in fruits; subsequently, the order of transcription level decrease was stems > roots > leaves. The cloning and characterization of RvSTR give a new STR sequence involved in TIA biosynthesis of R. verticillata, and provide a candidate gene for metabolic engineering of the TIA pathway in R. verticillata. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Molecular cloning and characterization of the Strictosidine synthase Gene from Rauwolfia verticillata

Loading next page...
 
/lp/springer_journal/molecular-cloning-and-characterization-of-the-strictosidine-synthase-bJ2mr0cb6C
Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2008 by MAIK Nauka
Subject
Life Sciences; Plant Sciences ; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443708050117
Publisher site
See Article on Publisher Site

Abstract

The strictosidine synthase (STR, EC 4.3.3.2) catalyzes the condensation of tryptamine and secologanin to form strictosidine, which is the universal precursor for a wide range of pharmaceutical terpenoid indole alkaloids (TIAs). The full-length cDNA encoding STR was cloned and characterized from Rauwolfia verticillata a Chinese native plant producing TIAs, such as reserpine and ajmalicine. The new cDNA was designed as RvSTR and submitted to GenBank to get an accession number DQ017054. The full-length cDNA of RvSTR was 1211 bp containing a 1035-bp open reading frame encoding a deduced 344-amino-acid polypeptide with a calculated mol wt of 38.2 kD and an isoelectric point of 5.19. Comparative and bioinformatic analysis revealed that RvSTR showed a higher similarity to STRs from Apocynaceae species, including Catharanthus roseus and Ophiorrhiza pumila, but a relatively lower similarity to other plant STRs. The unique essential catalytic residue Glu-309 was conserved in all alignment plant species. The phylogenetic analysis revealed that STRs were divided into two groups, including plant and bacterial enzymes. The tissue expression pattern analysis indicated that RvSTR expression could be detected in all tested organs of R. verticillata, including roots, stems, leaves, fruits, and flowers. The lowest transcription level was observed in flowers and the highest was found in fruits; subsequently, the order of transcription level decrease was stems > roots > leaves. The cloning and characterization of RvSTR give a new STR sequence involved in TIA biosynthesis of R. verticillata, and provide a candidate gene for metabolic engineering of the TIA pathway in R. verticillata.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Aug 30, 2008

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off