Molecular cloning and characterization of a TRANSPORT INHIBITOR RESPONSE 1 (TIR1) from Nicotiana tabacum

Molecular cloning and characterization of a TRANSPORT INHIBITOR RESPONSE 1 (TIR1) from Nicotiana... The full-length cDNA encoding a TRANSPORT INHIBITOR RESPONSE 1 (TIR1) protein, designated NtTIR1, was isolated for the first time from Nicotiana tabacum by the rapid amplification of cDNA ends (RACE) method. NtTIR1 contained a 1746-bp open reading frame encoding 581 amino acids. The deduced NtTIR1 protein, which showed high identity to TIR1 protein of other dicotyledonous plants, had a calculated mol wt of 65.2 kD and a theoretical pI value of 6.02 and was predicted to possess an F-box domain. Bioinformatic analyses revealed that the deduced NtTIR1 contained three transmembrane domains, and the predicted 3D model of NtTIR1 had a typical spatial structure of the TIR1 protein from Arabidopsis thaliana. Transcription pattern analysis revealed that the transcription of NtTIR1 was induced by IAA and ABA. Cloning of the NtTIR1 gene will enable us to further understand the molecular organization of the TIR1 and its possible function in the tobacco. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Molecular cloning and characterization of a TRANSPORT INHIBITOR RESPONSE 1 (TIR1) from Nicotiana tabacum

Loading next page...
 
/lp/springer_journal/molecular-cloning-and-characterization-of-a-transport-inhibitor-qTV9ubz2Tt
Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2011 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Sciences ; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443711010213
Publisher site
See Article on Publisher Site

Abstract

The full-length cDNA encoding a TRANSPORT INHIBITOR RESPONSE 1 (TIR1) protein, designated NtTIR1, was isolated for the first time from Nicotiana tabacum by the rapid amplification of cDNA ends (RACE) method. NtTIR1 contained a 1746-bp open reading frame encoding 581 amino acids. The deduced NtTIR1 protein, which showed high identity to TIR1 protein of other dicotyledonous plants, had a calculated mol wt of 65.2 kD and a theoretical pI value of 6.02 and was predicted to possess an F-box domain. Bioinformatic analyses revealed that the deduced NtTIR1 contained three transmembrane domains, and the predicted 3D model of NtTIR1 had a typical spatial structure of the TIR1 protein from Arabidopsis thaliana. Transcription pattern analysis revealed that the transcription of NtTIR1 was induced by IAA and ABA. Cloning of the NtTIR1 gene will enable us to further understand the molecular organization of the TIR1 and its possible function in the tobacco.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Jan 8, 2011

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from Google Scholar, PubMed
Create lists to organize your research
Export lists, citations
Read DeepDyve articles
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off