Molecular cloning and characterization of a phosphoinositide-specific phospholipase C from Torenia fournieri

Molecular cloning and characterization of a phosphoinositide-specific phospholipase C from... A full-length gene encoding phosphoinositide-specific phospholipase C (PI-PLC), named TfPLC2, was cloned from Torenia fournieri by RT-PCR and RACE, using the designed degenerate primers. The TfPLC2 gene is a 2087-bp-long sequence including one open reading frame encoding a polypeptide of 601 amino acids. Northern blot analysis showed that the TfPLC2 gene was expressed predominantly in leaves and stems and at very low levels in other tissues. The expression of TfPLC2 was strongly induced under the conditions of high salt or ABA treatment. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Molecular cloning and characterization of a phosphoinositide-specific phospholipase C from Torenia fournieri

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2008 by MAIK Nauka
Subject
Life Sciences; Plant Sciences ; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443708030151
Publisher site
See Article on Publisher Site

Abstract

A full-length gene encoding phosphoinositide-specific phospholipase C (PI-PLC), named TfPLC2, was cloned from Torenia fournieri by RT-PCR and RACE, using the designed degenerate primers. The TfPLC2 gene is a 2087-bp-long sequence including one open reading frame encoding a polypeptide of 601 amino acids. Northern blot analysis showed that the TfPLC2 gene was expressed predominantly in leaves and stems and at very low levels in other tissues. The expression of TfPLC2 was strongly induced under the conditions of high salt or ABA treatment.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: May 15, 2008

References

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