Arch Virol (2009) 154:541–543 DOI 10.1007/s00705-009-0314-z ANNOTATED SEQUENCE RECORD Molecular characterization of two alphacryptovirus dsRNAs isolated from Daucus carota Joerg Willenborg Æ Wulf Menzel Æ Heinrich-Josef Vetten Æ Edgar Maiss Received: 7 November 2008 / Accepted: 22 December 2008 / Published online: 23 January 2009 Springer-Verlag 2009 Introduction infecting plant cryptic virus. While VCV and WCCV occur in plants of the family Fabaceae, this is a report of a genome Although numerous cryptic viruses have been described sequence of an alphacryptovirus infecting a host plant of the since the late 60s , the genomes of only two plant- family Apiaceae. infecting cryptic viruses, namely vicia cryptic virus (VCV) and white clover cryptic virus (WCCV), have been com- Provenance of the virus material pletely sequenced and published to date [3–5]. Plant cryptic viruses belong either to the genus Alphacryptovirus or In order to clone the viral genome, double-stranded RNAs Betacryptovirus within the family Partitiviridae , were extracted from leaves of a ﬁeld-grown carrot plant whereas fungus-infecting viruses of this family are members according to the protocol of Dodds et al. . The ﬁrst cDNA of the genus Partitivirus. The genome of alphacryptoviruses clones obtained by random PCR approaches such as
Archives of Virology – Springer Journals
Published: Mar 1, 2009
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera