Molecular characterization of two alphacryptovirus dsRNAs isolated from Daucus carota

Molecular characterization of two alphacryptovirus dsRNAs isolated from Daucus carota Arch Virol (2009) 154:541–543 DOI 10.1007/s00705-009-0314-z ANNOTATED SEQUENCE RECORD Molecular characterization of two alphacryptovirus dsRNAs isolated from Daucus carota Joerg Willenborg Æ Wulf Menzel Æ Heinrich-Josef Vetten Æ Edgar Maiss Received: 7 November 2008 / Accepted: 22 December 2008 / Published online: 23 January 2009 Springer-Verlag 2009 Introduction infecting plant cryptic virus. While VCV and WCCV occur in plants of the family Fabaceae, this is a report of a genome Although numerous cryptic viruses have been described sequence of an alphacryptovirus infecting a host plant of the since the late 60s [15], the genomes of only two plant- family Apiaceae. infecting cryptic viruses, namely vicia cryptic virus (VCV) and white clover cryptic virus (WCCV), have been com- Provenance of the virus material pletely sequenced and published to date [3–5]. Plant cryptic viruses belong either to the genus Alphacryptovirus or In order to clone the viral genome, double-stranded RNAs Betacryptovirus within the family Partitiviridae [11], were extracted from leaves of a field-grown carrot plant whereas fungus-infecting viruses of this family are members according to the protocol of Dodds et al. [9]. The first cDNA of the genus Partitivirus. The genome of alphacryptoviruses clones obtained by random PCR approaches such as http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Molecular characterization of two alphacryptovirus dsRNAs isolated from Daucus carota

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Publisher
Springer Vienna
Copyright
Copyright © 2009 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-009-0314-z
Publisher site
See Article on Publisher Site

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