Arch Virol (2004) 149: 1721–1732
Molecular characterization of tomato-infecting
begomoviruses in Yunnan, China
Z. H. Li, X. P. Zhou, X. Zhang, and Y. Xie
Institute of Biotechnology, Zhejiang University, Hangzhou, P.R. China
Received October 1, 2003; accepted March 8, 2004
Published online April 27, 2004
Summary. The importance of diseases of tomato caused by begomoviruses is
increasing worldwide. Here, we report that several begomoviruses are associated
with tomato leaf curl disease inYunnan province, China. 14 tomato samples show-
ing leaf curl symptoms were collected in three districts inYunnan, and they fell into
four groups according to their reaction with a panel of 16 monoclonal antibodies in
TAS-ELISA. Comparison of partial DNA-A sequences ampliﬁed with degenerate
primers conﬁrmed the existence of several types of begomoviruses. The complete
DNA-A sequences of 4 isolates (Y25, Y41, Y72, Y161), corresponding to the
four groups, were determined. Sequence comparisons and phylogenetic analysis
revealed that they corresponded to four previously identiﬁed begomoviruses.
Groups I, II and IV are most closely related to Tomato yellow leaf curl China virus
(TYLCCNV), Tobacco curly shoot virus (TbCSV) and Tobacco leaf curl Yunnan
virus (TbLCYNV), respectively, while Group III shows close relationships with
Tomato yellow leaf curl Thailand virus (TYLCTHV). In addition, all isolates in
Groups I and III were found to be associated with DNAβ molecules, while satellite
DNA was not found in virus isolates in Groups II and IV. The complete DNAβ
sequences of three isolates from Group III (Y72, Y77, Y79) were determined.
Sequence analysis showed that Y72β, Y77β and Y79β seem to be different from
other characterised DNAβ, sharing the highest nucleotide sequence identity with
DNAβ of TbCSV.
Geminiviruses are plant viruses that are characterized by having small circular
single-stranded DNA (ssDNA) genomes, encapsidated in twinned icosahedral
The GenBank accession numbers of the sequences reported in this paper areAJ514313-21,
AJ566744-48, AJ457985, AJ457986 and AJ495812.