Molecular characterization of three non-functional S-haplotypes in sour cherry (Prunus cerasus)

Molecular characterization of three non-functional S-haplotypes in sour cherry (Prunus cerasus) Tetraploid sour cherry (Prunus cerasus) exhibits a genotype-dependent loss of gametophytic self-incompatibility that is caused by the accumulation of non-functional S-haplotypes with disrupted pistil component (stylar-S) and/or pollen component (pollen-S) function. Genetic studies using diverse sour cherry germplasm identified non-functional S-haplotypes for which an equivalent wild-type S-haplotype was present in sweet cherry (Prunus avium), a diploid progenitor of sour cherry. In all cases, the non-functional S-haplotype resulted from mutations affecting the stylar component S-RNase or Prunus pollen component S-haplotype-specific F-box protein (SFB). This study determines the molecular bases of three of these S-haplotypes that confer unilateral incompatibility, two stylar-part mutants (S 6m2 and S 13m ) and one pollen-part mutant (S 13 ′). Compared to their wild-type alleles, S 6m2 -RNase has a 1 bp deletion, S 13m -RNase has a 23 bp deletion and SFB 13 ′ has a 1 bp substitution that lead to premature stop codons. Transcripts were identified for these three alleles, S 6m2 -RNase, S 13m -RNase, and SFB 13 ′, however, these transcripts presumably result in altered proteins with a resulting loss of activity. Our characterization of natural pollen-part and stylar-part mutants in sour cherry along with other natural S-haplotype mutants identified in Prunus supports the view that loss of pollen specificity and stylar rejection evolve independently and are caused by structural alterations affecting the S-haplotype. The prevalence of non-functional S-haplotypes in sour cherry but not in sweet cherry (a diploid) suggests that polyploidization and gene duplication were indirectly responsible for the dysfunction of some S-haplotypes and the emergence of self-compatibility in sour cherry. This resembles the specific mode of evolution in yeast where accelerated evolution occurred to one member of the duplicated gene pair. Plant Molecular Biology Springer Journals

Molecular characterization of three non-functional S-haplotypes in sour cherry (Prunus cerasus)

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Kluwer Academic Publishers
Copyright © 2006 by Springer Science+Business Media B.V.
Life Sciences; Plant Pathology; Plant Sciences ; Plant Sciences
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