Molecular characterization of three differentially expressed members of the Camptotheca acuminata 3-hydroxy-3-methylglutaryl CoA reductase (HMGR) gene family

Molecular characterization of three differentially expressed members of the Camptotheca acuminata... Camptotheca acuminata is a Chinese tree that produces the anti-cancer monoterpenoid indole alkaloid camptothecin (CPT). 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) supplies mevalonate for the terpenoid moiety of CPT and its hydroxylated derivative 10-hydroxycamptothecin (10-OH-CPT). We previously described the isolation of a gene encoding HMGR from C. acuminata (hmg1) and analyzed its expression in transgenic tobacco [6]. Here, we report on the isolation of genomic (hmg2) and cDNA (hmg3) clones representing two additional HMGR gene family members and characterize the expression of all three genes in C. acuminata. Transcript levels for two family members were highest in the shoot apex, dry seeds (hmg1), and bark (hmg3) which are the tissues containing the highest levels of CPT and 10-OH-CPT respectively. Levels of hmg3 mRNA also correlated with the accumulation of 10-OH-CPT during germination. In C. acuminata leaf disks, hmg1 mRNA increased in response to wounding, and this induction was suppressed by methyl jasmonate (MeJA), in agreement with results previously obtained in transgenic tobacco [6]. In contrast, wounding and MeJA did not affect hmg2 or hmg3 transcript levels in C. acuminata. These results show that members of the C. acuminata HMGR gene family are differentially expressed in various tissues under different physiological conditions which may contribute to the regulation of monoterpenoid indole alkaloid synthesis in this species. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Molecular characterization of three differentially expressed members of the Camptotheca acuminata 3-hydroxy-3-methylglutaryl CoA reductase (HMGR) gene family

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Publisher
Kluwer Academic Publishers
Copyright
Copyright © 1997 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1005866813347
Publisher site
See Article on Publisher Site

Abstract

Camptotheca acuminata is a Chinese tree that produces the anti-cancer monoterpenoid indole alkaloid camptothecin (CPT). 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) supplies mevalonate for the terpenoid moiety of CPT and its hydroxylated derivative 10-hydroxycamptothecin (10-OH-CPT). We previously described the isolation of a gene encoding HMGR from C. acuminata (hmg1) and analyzed its expression in transgenic tobacco [6]. Here, we report on the isolation of genomic (hmg2) and cDNA (hmg3) clones representing two additional HMGR gene family members and characterize the expression of all three genes in C. acuminata. Transcript levels for two family members were highest in the shoot apex, dry seeds (hmg1), and bark (hmg3) which are the tissues containing the highest levels of CPT and 10-OH-CPT respectively. Levels of hmg3 mRNA also correlated with the accumulation of 10-OH-CPT during germination. In C. acuminata leaf disks, hmg1 mRNA increased in response to wounding, and this induction was suppressed by methyl jasmonate (MeJA), in agreement with results previously obtained in transgenic tobacco [6]. In contrast, wounding and MeJA did not affect hmg2 or hmg3 transcript levels in C. acuminata. These results show that members of the C. acuminata HMGR gene family are differentially expressed in various tissues under different physiological conditions which may contribute to the regulation of monoterpenoid indole alkaloid synthesis in this species.

Journal

Plant Molecular BiologySpringer Journals

Published: Sep 29, 2004

References

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