Molecular characterization of the guinea-pig cytomegalovirus glycoprotein L gene

Molecular characterization of the guinea-pig cytomegalovirus glycoprotein L gene Although the guinea pig cytomegalovirus (GPCMV) model is well suited to the study of vaccines for prevention of congenital CMV infection, there has been limited molecular characterization of GPCMV glycoproteins. Since the in vivo co-expression of the human cytomegalovirus (HCMV) glycoprotein H (gH, gpUL75) with glycoprotein L (gL, gpUL115) may have relevance to CMV vaccine studies, these experiments were undertaken to test whether the GPCMV encodes a gL homolog. Sequencing of the Eco R I “G” fragment of the GPCMV genome identified an open reading frame (ORF) of 774 nucleotides capable of encoding a protein of 258 amino acids. Computer matrix analyses demonstrated identity between this ORF and the gL coding sequences of other betaherpesviruses. Sequence analysis also identified an ORF with identity to the HCMV uracil DNA glycosylase (UDG, UL114 gene). The GPCMV gL ORF encodes 6 cysteine residues, contains 3 potential N -linked glycosylation sites, and has a predicted M r of 29.7 kDa. Northern blot studies identified an abundant 2.7 kb “early” transcript from infected cells, the putative gL message. In vitro translation of gL mRNA in reticulocyte lysate resulted in synthesis of 30 kDa polypeptide. A polyclonal antiserum was raised against a gL/glutathione-S-transferase fusion protein generated in E. coli using the pGEX expression system. This antibody identified a 40-kDa virion-associated protein, the putative GPCMV gL, in immunoblot assays. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Molecular characterization of the guinea-pig cytomegalovirus glycoprotein L gene

Loading next page...
 
/lp/springer_journal/molecular-characterization-of-the-guinea-pig-cytomegalovirus-hq8PvRVOvO
Publisher
Springer-Verlag
Copyright
Copyright © Wien by 1999 Springer-Verlag/
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050050517
Publisher site
See Article on Publisher Site

Abstract

Although the guinea pig cytomegalovirus (GPCMV) model is well suited to the study of vaccines for prevention of congenital CMV infection, there has been limited molecular characterization of GPCMV glycoproteins. Since the in vivo co-expression of the human cytomegalovirus (HCMV) glycoprotein H (gH, gpUL75) with glycoprotein L (gL, gpUL115) may have relevance to CMV vaccine studies, these experiments were undertaken to test whether the GPCMV encodes a gL homolog. Sequencing of the Eco R I “G” fragment of the GPCMV genome identified an open reading frame (ORF) of 774 nucleotides capable of encoding a protein of 258 amino acids. Computer matrix analyses demonstrated identity between this ORF and the gL coding sequences of other betaherpesviruses. Sequence analysis also identified an ORF with identity to the HCMV uracil DNA glycosylase (UDG, UL114 gene). The GPCMV gL ORF encodes 6 cysteine residues, contains 3 potential N -linked glycosylation sites, and has a predicted M r of 29.7 kDa. Northern blot studies identified an abundant 2.7 kb “early” transcript from infected cells, the putative gL message. In vitro translation of gL mRNA in reticulocyte lysate resulted in synthesis of 30 kDa polypeptide. A polyclonal antiserum was raised against a gL/glutathione-S-transferase fusion protein generated in E. coli using the pGEX expression system. This antibody identified a 40-kDa virion-associated protein, the putative GPCMV gL, in immunoblot assays.

Journal

Archives of VirologySpringer Journals

Published: Mar 1, 1999

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from Google Scholar, PubMed
Create lists to organize your research
Export lists, citations
Read DeepDyve articles
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off