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S. Dunn, R. Ward, M. McNeal, T. Cross, H. Greenberg (1993)
Identification of a new neutralization epitope on VP7 of human serotype 2 rotavirus and evidence for electropherotype differences caused by single nucleotide substitutions.Virology, 197 1
越村 裕美 (2001)
The relative frequencies of G serotypes of rotaviruses recovered from hospitalized children with diarrhea : A 10-year survey(1987-1996)in Japan with a review of globally collected data
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Similarity of the VP4 protein of human rotavirus strain 116E to that of the bovine B223 strain.Virology, 194 1
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U. Laemmli (1970)
Cleavage of structural proteins duringNature
O. Nakagomi, T. Nakagomi, K. Akatani, N. Ikegami (1989)
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M. Ramachandran, B. Das, A. Vij, R. Kumar, S. Bhambal, Nirmala Kesari, Harish Rawat, Lilita Bahl, Savitri Thakur, P. Woods, R. Glass, M. Bhan, J. Gentsch (1996)
Unusual diversity of human rotavirus G and P genotypes in IndiaJournal of Clinical Microbiology, 34
B. Das, J. Gentsch, Y. Hoshino, S. Ishida, O. Nakagomi, M. Bhan, R. Kumar, R. Glass (1993)
Characterization of the G serotype and genogroup of New Delhi newborn rotavirus strain 116E.Virology, 197 1
R. Espejo, F. Puerto (1984)
Shifts in the electrophoretic pattern on the RNA genome of rotaviruses under different electrophoretic conditions.Journal of virological methods, 8 4
T. Nakagomi, K. Akatani, N. Ikegami, N. Katsushima, O. Nakagomi (1988)
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M. Watanabe, T. Nakagomi, Y. Koshimura, O. Nakagomi (2001)
Direct evidence for genome segment reassortment between concurrently-circulating human rotavirus strainsArchives of Virology, 146
S. Gunasena, O. Nakagomi, Y. Isegawa, E. Kaga, T. Nakagomi, A. Steele, J. Flores, S. Ueda (1993)
Relative frequency of VP4 gene alleles among human rotaviruses recovered over a 10-year period (1982-1991) from Japanese children with diarrheaJournal of Clinical Microbiology, 31
Barbara Coulson (1987)
Variation in neutralization epitopes of human rotaviruses in relation to genomic RNA polymorphism.Virology, 159 2
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Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimensJournal of Clinical Microbiology, 28
Vivek Jain, B. Das, M. Bhan, R. Glass, J. Gentsch (2001)
Great Diversity of Group A Rotavirus Strains and High Prevalence of Mixed Rotavirus Infections in IndiaJournal of Clinical Microbiology, 39
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Development of rotavirus molecular epidemiology: electropherotyping.Archives of virology. Supplementum, 12
The literature is conflicting whether or not rotavirus strains with different G serotype have an identical electropherotype. This is a contentious but an important issue because large parts of molecular epidemiological studies of rotaviruses have been based on the conception that a single strain of rotavirus can be defined by a single electropherotype. Here, we examined in detail by reverse-transcription PCR genotyping, electropherotyping, sequencing, and genogrouping using RNA--RNA hybridization three human rotavirus strains isolated in India that had apparently identical electropherotypes although one strain was typed as P(4), G3 while the other two typed as P(4), G2. These three strains showed an identical electropherotype on 7.5% and 12.5% polyacrylamide gels, but co-electrophoresis on a 10% gel demonstrated that segment 8 of the P(4), G3 strain migrated more slowly than the cognate segment of the P(4), G2 strains. Genogrouping assay and nucleotide sequencing provided evidence for the hypothesis that the P(4), G3 stain was an intergenogroup reassortant in which a P(4), G2 strain of the DS-1 genogroup had acquired the VP7 gene from an yet-unidentified concurrently circulating G3 strain. While electropherotyping remains a valuable asset for molecular epidemiology of rotaviruses, this study underscores the importance of co-electrophoresis under different electrophoretic conditions when pinpointing subtle differences.
Archives of Virology – Springer Journals
Published: Oct 1, 2002
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