Molecular characterization of Lolium latent virus, proposed type member of a new genus in the family Flexiviridae

Molecular characterization of Lolium latent virus, proposed type member of a new genus in the... Lolium latent virus (LoLV) was recently detected in the USA for the first time in ryegrass hybrids ( Lolium perenne × Lolium multiflorum ). The genome of one USA isolate, LoLV-US1, has now been fully sequenced. The positive strand genomic RNA is 7674 nucleotides (nt) long and is organized in five open reading frames (ORFs) encoding the replication-associated protein, the movement-associated triple gene block proteins and the coat protein (CP). The genome organization is similar to that of viruses in the genera Potexvirus and Foveavirus ; however, analysis of the complete LoLV genomic sequence, phylogenetic analyses of the deduced amino acid (aa) sequences of the polymerase and the CP, presence of a putative ORF 6, and the in vivo detection of two CPs in equimolar amounts, highlight features peculiar to LoLV. These characteristics indicate that LoLV forms a monotypic group separate from existing genera and unassigned species within the family Flexiviridae , for which we propose the genus name Lolavirus. One-step RT-PCR was developed for quick and reliable LoLV detection. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Molecular characterization of Lolium latent virus, proposed type member of a new genus in the family Flexiviridae

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Publisher
Springer Vienna
Copyright
Copyright © 2008 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-008-0108-8
Publisher site
See Article on Publisher Site

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