Dinitroaniline herbicides are antimicrotubule drugs that bind to tubulins and inhibit polymerization. As a result of repeated application of dinitroaniline herbicides, resistant biotypes of goosegrass (Eleusine indica) developed in previously susceptible wild-type populations. We have previously reported that α-tubulin missense mutations correlate with dinitroaniline response phenotypes (Drp) (Plant Cell 10: 297–308, 1998). In order to ascertain associations of other tubulins with dinitroaniline resistance, four β-tubulin cDNA classes (designated TUB1, TUB2, TUB3, and TUB4) were isolated from dinitroaniline-susceptible and -resistant biotypes. Sequence analysis of the four β-tubulin cDNA classes identified no missense mutations. Identified nucleotide substitutions did not result in amino acid replacements. These results suggest that the molecular basis of dinitroaniline resistance in goosegrass differs from those of colchicine/dinitroaniline cross-resistant Chlamydomonas reinhardtii and benzimidazole-resistant fungi and yeast. Expression of the four β-tubulins was highest in inflorescences. This is in contrast to α-tubulin TUA1 that is expressed predominantly in roots. Collectively, these results imply that β-tubulin genes are not associated with dinitroaniline resistance in goosegrass. Phylogenetic analysis of the four β-tubulins, together with three α-tubulins, suggests that the resistant biotype developed independently in multiple locations rather than spreading from one location.
Plant Molecular Biology – Springer Journals
Published: Sep 29, 2004
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