Molecular characterization of Citrus tristeza virus isolates from the Northeastern Himalayan region of India

Molecular characterization of Citrus tristeza virus isolates from the Northeastern Himalayan... Citrus tristeza virus (CTV) isolates from the Darjeeling hills of the Northeastern Himalayan region of India were characterized by biological indexing, multiple molecular marker (MMM) analysis, heteroduplex mobility assay (HMA) and sequence analysis. Variability was studied using the CP gene and a 5′ ORF1a fragment of the CTV genome. HMA and sequence analysis of the 5′ ORF1a fragment classified Darjeeling isolates into two groups, whereas CP gene analysis provided evidence for three different groups. Darjeeling CTV isolates shared nucleotide sequence identities of 89–97 and 91–92% in the 5′ ORF1a fragment and CP gene, respectively, suggesting extensive diversity among CTV isolates from this Indian region. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Molecular characterization of Citrus tristeza virus isolates from the Northeastern Himalayan region of India

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Publisher
Springer Vienna
Copyright
Copyright © 2010 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-010-0602-7
Publisher site
See Article on Publisher Site

Abstract

Citrus tristeza virus (CTV) isolates from the Darjeeling hills of the Northeastern Himalayan region of India were characterized by biological indexing, multiple molecular marker (MMM) analysis, heteroduplex mobility assay (HMA) and sequence analysis. Variability was studied using the CP gene and a 5′ ORF1a fragment of the CTV genome. HMA and sequence analysis of the 5′ ORF1a fragment classified Darjeeling isolates into two groups, whereas CP gene analysis provided evidence for three different groups. Darjeeling CTV isolates shared nucleotide sequence identities of 89–97 and 91–92% in the 5′ ORF1a fragment and CP gene, respectively, suggesting extensive diversity among CTV isolates from this Indian region.

Journal

Archives of VirologySpringer Journals

Published: Jun 1, 2010

References

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