Molecular characterization of an atypical Old World strain of Peanut stunt virus

Molecular characterization of an atypical Old World strain of Peanut stunt virus Northern blot analyses of RNAs from an Iranian strain of Peanut stunt virus (PSV-I Cucumovirus ) using cloned cDNA probes to the genomic RNAs from two PSV strains, PSV-ER (subgroup I) and PSV-W (subgroup II), indicated that PSV-I RNA-3 is derived from a subgroup II strain. No hybridization signals, however, were detected with PSV-I RNAs 1 and 2 suggesting they are distinct from both subgroups I and II. Nucleotide (nt) sequence analysis of cloned cDNA fragments (1–1.5 kbp in size) representing PSV-I RNAs 1 and 2 showed that PSV-I is more closely related to subgroup II strains than to any other sequenced cucumovirus. The percent nt identity between RNA-1 sequences from PSV-I and PSV-ER or between PSV-I and PSV-W were 79.1% and 88.8%, respectively. The corresponding values for RNA-2 were 77.5% and 86.7%. Sequence comparison analyses of deduced amino acid sequence of cloned partial sequences of PSV-I RNAs 1 and 2 indicated that PSV-I 1a and 2a proteins are most closely related to those of subgroup II strains (93% identity). PSV-I supported the replication and encapsidation of PSV G-satellite RNA (G-satRNA), but not cucumber mosaic virus WL1-satRNA. PSV-I may be perceived as an Old World derivative of subgroup II strains, or it may represent a natural reassortment between a subgroup II (RNA-3) and an as yet uncharacterized subgroup of PSV strains (RNAs 1 and 2). Archives of Virology Springer Journals

Molecular characterization of an atypical Old World strain of Peanut stunt virus

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