Molecular characterization of a novel victorivirus from the entomopathogenic fungus Beauveria bassiana

Molecular characterization of a novel victorivirus from the entomopathogenic fungus Beauveria... New Zealand isolates of the entomopathogenic fungus Beauveria were examined for the presence of dsRNAs and virus-like particles. Seven out of nine isolates contained one or more high-molecular-weight dsRNAs and all seven contained isometric virus particles ranging in size from 30 to 50 nm. B. bassiana isolate ICMP#6887 contained a single dsRNA band of ~6 kb and isometric virus-like particles of ~50 nm in diameter. Sequencing revealed that the virus from ICMP#6887 had a genome of 5,327 nt with two overlapping ORFs coding for a putative coat protein (CP) and an RNA-dependent RNA-polymerase (RdRp). The sequence showed a highest CP identity of 58.3 % to Tolypocladium cylindrosporum virus 1 (TcV1) and a highest RdRp identity of 48.8 % to Sphaeropsis sapinea RNA virus 1 (SsRV1). Since both TcV1 and SsRV1 belong to the genus Victorivirus , the new virus from B. bassiana ICMP#6887 was tentatively assigned the name Beauveria bassiana victorivirus 1 (BbVV1-6887). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Molecular characterization of a novel victorivirus from the entomopathogenic fungus Beauveria bassiana

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Publisher
Springer Vienna
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1938-6
Publisher site
See Article on Publisher Site

Abstract

New Zealand isolates of the entomopathogenic fungus Beauveria were examined for the presence of dsRNAs and virus-like particles. Seven out of nine isolates contained one or more high-molecular-weight dsRNAs and all seven contained isometric virus particles ranging in size from 30 to 50 nm. B. bassiana isolate ICMP#6887 contained a single dsRNA band of ~6 kb and isometric virus-like particles of ~50 nm in diameter. Sequencing revealed that the virus from ICMP#6887 had a genome of 5,327 nt with two overlapping ORFs coding for a putative coat protein (CP) and an RNA-dependent RNA-polymerase (RdRp). The sequence showed a highest CP identity of 58.3 % to Tolypocladium cylindrosporum virus 1 (TcV1) and a highest RdRp identity of 48.8 % to Sphaeropsis sapinea RNA virus 1 (SsRV1). Since both TcV1 and SsRV1 belong to the genus Victorivirus , the new virus from B. bassiana ICMP#6887 was tentatively assigned the name Beauveria bassiana victorivirus 1 (BbVV1-6887).

Journal

Archives of VirologySpringer Journals

Published: Jun 1, 2014

References

  • Victorivirus , a new genus of fungal viruses in the family Totiviridae
    Ghabrial, SA; Nibert, ML
  • Interactions between fungal pathogens and insect hosts
    Hajek, AE; St. Leger, RJ
  • Mycoviruses infecting the endophytic and entomopathogenic fungus Tolypocladium cylindrosporum
    Herrero, N; Zabalgogeazcoa, I
  • Mycoviruses are common among different species of endophytic fungi of grasses
    Herrero, N; Sánchez Márquez, S; Zabalgogeazcoa, I
  • Genome characterization of a flexuous rod-shaped mycovirus, Botrytis virus X, reveals high amino acid identity to genes from plant potex-like viruses
    Howitt, RL; Beever, RE; Pearson, MN; Forster, RL
  • A heuristic approach for detecting RNA H-type pseudoknots
    Huang, CH; Lu, CL; Chiu, HT

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