Molecular characterization of a novel luteovirus from peach identified by high-throughput sequencing

Molecular characterization of a novel luteovirus from peach identified by high-throughput sequencing Contigs with sequence homologies to cherry-associated luteovirus were identified by high-throughput sequencing analysis in two peach accessions. Complete genomic sequences of the two isolates of this virus were determined to be 5,819 and 5,814 nucleotides long, respectively. The genome of the new virus is typical of luteoviruses, containing eight open reading frames in a very similar arrangement. Its genomic sequence is 58-74% identical to those of other members of the genus Luteovirus. These sequences thus belong to a new virus, which we have named “peach-associated luteovirus”. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Molecular characterization of a novel luteovirus from peach identified by high-throughput sequencing

Molecular characterization of a novel luteovirus from peach identified by high-throughput sequencing

Arch Virol (2017) 162:2903–2905 DOI 10.1007/s00705-017-3388-z ANNOTATED SEQUENCE RECORD Molecular characterization of a novel luteovirus from peach identified by high-throughput sequencing 1,2 1 3 4 1 • • • • L.-P. Wu H.-W. Liu M. Bateman Z. Liu R. Li Received: 9 March 2017 / Accepted: 12 April 2017 / Published online: 26 May 2017 Springer-Verlag Wien 2017 Abstract Contigs with sequence homologies to cherry- are comprised of 25- to 30-nm isometric particles with a associated luteovirus were identified by high-throughput single strand of plus-sense RNA from 5.3 to 5.9 kb, and sequencing analysis in two peach accessions. Complete are transmitted by aphids in a circulative, non-persistent genomic sequences of the two isolates of this virus were manner [2, 3]. Three luteoviruses, rose spring dwarf-as- determined to be 5,819 and 5,814 nucleotides long, sociated virus (RSDaV), nectarine stem pitting-associated respectively. The genome of the new virus is typical of virus (NSPaV), and cherry-associated luteovirus luteoviruses, containing eight open reading frames in a (ChALV), have recently been reported in woody plants very similar arrangement. Its genomic sequence is 58-74% [4–6]. identical to those of other members of the genus Lu- Six Prunus accessions used in this study were imported teovirus. These sequences thus belong to a new virus, from Europe. They were established by grafting scion buds which we have named ‘‘peach-associated luteovirus’’. onto peach rootstock. The accessions were also grafted onto eight woody indicator plants. Total RNA was extracted from leaves of each accession and pooled into Peaches (Prunus persica) are a globally important stone two samples, P5 and P6. Each of the two pooled samples fruit belonging to the genus Prunus of the family Rosa- represented two apricot accessions and one peach acces- ceae. A number of viruses and virus-like pathogens infect sion. The samples were...
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Publisher
Springer Vienna
Copyright
Copyright © 2017 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-017-3388-z
Publisher site
See Article on Publisher Site

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