Molecular characterization of a new soybean-infecting member of the genus Nepovirus identified by high-throughput sequencing

Molecular characterization of a new soybean-infecting member of the genus Nepovirus identified by... The complete nucleotide sequence of a new soybean-infecting member of the genus Nepovirus (provisionally named “soybean latent spherical virus” [SLSV]) was identified by high-throughput sequencing of RNAs from soybean leaf samples from North Dakota, USA. The sequences of RNAs 1 (8,190 nt) and 2 (5,788 nt) were completed by rapid amplification of cDNA ends. Each contained a single long open reading frame and a 3’ nontranslated region of greater than 1,500 nt. The predicted amino acid sequences of the two ORFs were most closely related to nepoviruses in subgroup C. Full-length cDNAs of RNAs 1 and 2 were cloned and used to inoculate soybean plants, which did not display obvious symptoms. These results suggest that SLSV represents a new species in the genus Nepovirus. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Molecular characterization of a new soybean-infecting member of the genus Nepovirus identified by high-throughput sequencing

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Publisher
Springer Vienna
Copyright
Copyright © 2016 by Springer-Verlag Wien (Outside the USA)
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-016-3152-9
Publisher site
See Article on Publisher Site

Abstract

The complete nucleotide sequence of a new soybean-infecting member of the genus Nepovirus (provisionally named “soybean latent spherical virus” [SLSV]) was identified by high-throughput sequencing of RNAs from soybean leaf samples from North Dakota, USA. The sequences of RNAs 1 (8,190 nt) and 2 (5,788 nt) were completed by rapid amplification of cDNA ends. Each contained a single long open reading frame and a 3’ nontranslated region of greater than 1,500 nt. The predicted amino acid sequences of the two ORFs were most closely related to nepoviruses in subgroup C. Full-length cDNAs of RNAs 1 and 2 were cloned and used to inoculate soybean plants, which did not display obvious symptoms. These results suggest that SLSV represents a new species in the genus Nepovirus.

Journal

Archives of VirologySpringer Journals

Published: Dec 5, 2016

References

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