Molecular characterization of a new lytic bacteriophage isolated from cheese whey

Molecular characterization of a new lytic bacteriophage isolated from cheese whey In this study, we isolated and characterized a lytic Lactococcus lactis bacteriophage from the sera of a failed fermentation. The phage was isolated and cultured in L. lactis subsp. cremoris in M17 medium. The isolated bacteriophage was characterized by multiplex PCR, pulsed-field electrophoresis, DNA restriction digestion, analysis of the N-terminal sequence of the phage major structural protein, transmission electron microscopy and sequencing and analysis of a conserved fragment of its genome. Analysis of the viral genome indicates that its genome is composed of a DNA strand of approximately 48 kb in length, and PCR and microscopy confirmed that IL-P1 belongs to the group of 936-type phages in the family Siphoviridae , which is the most abundant type of lactococcal virus in dairy products worldwide. To our knowledge, this is the first report of a virus within this family that has a presumptive genome larger than 40 kb. Archives of Virology Springer Journals

Molecular characterization of a new lytic bacteriophage isolated from cheese whey

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Springer Vienna
Copyright © 2012 by Springer-Verlag Wien
Biomedicine; Virology; Infectious Diseases; Medical Microbiology
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