Molecular characterization of a Hungarian isolate of Tobacco necrosis virus A

Molecular characterization of a Hungarian isolate of Tobacco necrosis virus A Arch Virol (2010) 155:999–1001 DOI 10.1007/s00705-010-0660-x ANNOTATED SEQUENCE RECORD Molecular characterization of a Hungarian isolate of Tobacco necrosis virus A • • • Laszlo Krizbai Maria Nemeth Vivian Law • • • Christopher Reed Aniko Varga Rozalia Hangyal Delano James Received: 11 January 2010 / Accepted: 9 March 2010 / Published online: 3 April 2010 Springer-Verlag 2010 The genus Necrovirus, family Tombusviridae [8] contains co-infected with cymbidium ringspot virus [10]. Isolates of seven species, including Tobacco necrosis virus A (TNV-A), TNV-D have a genome size of 3,761–3,762 nucleotides and Tobacco necrosis virus D (TNV-D) [5]. (nt), with a 38-nt UTR leader sequence at the 5 terminus of Members of this genus are characterized by icosahedral the genome [2, 3, 10]. particles, approximately 28 nm in diameter, with a posi- TNV-A isolate Velence was first detected in Chenopo- tive-sense single-stranded RNA genome of approximately dium quinoa co-infected with apple chlorotic leaf spot 3.8–4.0 kb in size [8, 9]. The 5 terminus of the genome is virus (ACLSV) growing in a greenhouse in Velence, uncapped, and it lacks a poly(A) tail at the 3 end [8]. Some Hungary. C. quinoa plants were used as bio-indicators for of these viruses, including TNV-A Archives of Virology Springer Journals

Molecular characterization of a Hungarian isolate of Tobacco necrosis virus A

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Copyright © 2010 by Springer-Verlag
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
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