Molecular characterization and stress signaling of the Miscanthus sinensis MsCOMT gene by transient assay

Molecular characterization and stress signaling of the Miscanthus sinensis MsCOMT gene by... The full-length 1089-bp cDNA of caffeic acid 3-O-methyltransferase (COMT) was isolated from a Miscanthus sinensis cDNA library and was found to encode a 362-amino-acid-long protein. The analysis of the deduced tomato MsCOMT amino acid sequence revealed that it shares 98, 95, 92, and 80% sequence homologies with the respective proteins in Saccharum, Sorghum, maize, and Festuca arundinacea. The transcript level of the MsCOMT gene responded only to ABA signaling as compared to other environmental stressors such as mannitol, NaCl, and methyl viologen (MV) related to drought, salt, and ROS. However, MsCOMT expression decreased following the treatments with NaCl and MV. We analyzed NbPAL (Nicotiana benthamiana phenylalanine ammonia-lyase) gene expression in the leaves of Nicotiana benthamiana plants in response to the antisense MsCOMT-AS at the period ranging from 0 to 48 h. The PAL transcript level decreased when the antisense MsCOMT gene was transiently overexpressed. PR (Pathogenesis-Related) group genes led to a decrease in MsCOMT-AS gene expression in leaves. The NbAPX (Nicotiana benthamiana ascorbate peroxidase) expression level decreased following a decrease in NbPAL and PR group gene expression. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Molecular characterization and stress signaling of the Miscanthus sinensis MsCOMT gene by transient assay

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2012 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443712050135
Publisher site
See Article on Publisher Site

Abstract

The full-length 1089-bp cDNA of caffeic acid 3-O-methyltransferase (COMT) was isolated from a Miscanthus sinensis cDNA library and was found to encode a 362-amino-acid-long protein. The analysis of the deduced tomato MsCOMT amino acid sequence revealed that it shares 98, 95, 92, and 80% sequence homologies with the respective proteins in Saccharum, Sorghum, maize, and Festuca arundinacea. The transcript level of the MsCOMT gene responded only to ABA signaling as compared to other environmental stressors such as mannitol, NaCl, and methyl viologen (MV) related to drought, salt, and ROS. However, MsCOMT expression decreased following the treatments with NaCl and MV. We analyzed NbPAL (Nicotiana benthamiana phenylalanine ammonia-lyase) gene expression in the leaves of Nicotiana benthamiana plants in response to the antisense MsCOMT-AS at the period ranging from 0 to 48 h. The PAL transcript level decreased when the antisense MsCOMT gene was transiently overexpressed. PR (Pathogenesis-Related) group genes led to a decrease in MsCOMT-AS gene expression in leaves. The NbAPX (Nicotiana benthamiana ascorbate peroxidase) expression level decreased following a decrease in NbPAL and PR group gene expression.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Aug 16, 2012

References

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