Molecular characterization and multiple infections of rotavirus, norovirus, sapovirus, astrovirus and adenovirus in outpatients with sporadic gastroenteritis in Shanghai, China, 2010–2011

Molecular characterization and multiple infections of rotavirus, norovirus, sapovirus, astrovirus... Rotavirus (RV), norovirus (NoV), sapovirus (SaV), human astrovirus (HAstV) and human adenovirus (HAdV) are significant because they are the most common pathogens that cause diarrhea in young children. The aim of this study was to investigate the genetic characteristics and compare the roles of these five viruses in outpatient children with diarrhea in Shanghai. A total of 436 fecal samples were collected from pediatric patients with acute gastroenteritis from January 2010 to December 2011. The selected samples were subjected to reverse transcription PCR (RT-PCR) or PCR to detect and genotype RV, NoV, SaV, HAstV and HAdV. RV (43.3 %, 189/436) was the most prevalent virus, followed by NoV (28.9 %, 126/436), HAdV (7.1 %, 31/436). HAstV (1.8 %, 8/436) and SaV (0.5 %, 2/436). The percentage of multiple infection cases was 14.9 % (65/436), and RV + NoV was the predominant mixed infection. The RV genotype combinations of P(8)G3 (52/189, 27.5 %), P(8)G1 (51/189, 26.9 %) and P(8)G9 (48/189, 25.4 %) occurred most frequently. The predominant NoV genotype was GII.4 (73.0 %, 92/126), and the majority of GII.4 clustered as GII.4-2006b (65.2 %, 60/92). Two of the SaV cases were identified as GI.2 and GII.1. All HAstV-positive samples belonged to HAstV-1. The predominant HAdV type was HAdV-41 (45.2 %, 14/31). This study clearly shows the diversity of the viral causative agents of acute gastroenteritis in outpatient children in Shanghai, which will provide baseline information for future vaccination strategies and development in this area. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Molecular characterization and multiple infections of rotavirus, norovirus, sapovirus, astrovirus and adenovirus in outpatients with sporadic gastroenteritis in Shanghai, China, 2010–2011

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Publisher
Springer Vienna
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2387-1
Publisher site
See Article on Publisher Site

References

  • Viral gastroenteritis
    Kapikian, AZ
  • Etiology and epidemiology of viral diarrhea in children under the age of five hospitalized in Tianjin, China
    Ouyang, Y; Ma, H; Jin, M; Wang, X; Wang, J; Xu, L; Lin, S; Shen, Z; Chen, Z; Qiu, Z; Gao, Z; Peng, L; Li, J
  • An outbreak of adenovirus serotype 41 infection in infants and children with acute gastroenteritis in Maizuru City, Japan
    Shimizu, H; Phan, TG; Nishimura, S; Okitsu, S; Maneekarn, N; Ushijima, H
  • Existence of multiple outbreaks of viral gastroenteritis among infants in a day care center in Japan
    Akihara, S; Phan, TG; Nguyen, TA; Hansman, G; Okitsu, S; Ushijima, H
  • Molecular epidemiology of human calicivirus infections in children with acute diarrhea in Shanghai: a retrospective comparison between inpatients and outpatients treated between 2006 and 2011
    Lu, L; Zhong, H; Xu, M; Su, L; Cao, L; Dong, N; Xu, J
  • Noroviruses in children seen in a hospital for acute gastroenteritis in Finland
    Rasanen, S; Lappalainen, S; Salminen, M; Huhti, L; Vesikari, T
  • High prevalence of community-acquired norovirus gastroenteritis among hospitalized children: a prospective study
    Gonzalez-Galan, V; Sánchez-Fauqier, A; Obando, I; Montero, V; Fernandez, M; Torres, MJ; Neth, O; Aznar-Martin, J
  • Molecular epidemiology of adenovirus infection among infants and children with acute gastroenteritis in Dhaka City, Bangladesh
    Dey, SK; Shimizu, H; Phan, TG; Hayakawa, Y; Islam, A; Salim, AF; Khan, AR; Mizuguchi, M; Okitsu, S; Ushijima, H
  • Viral gastroenteritis in rotavirus negative hospitalized children <5 years of age from the independent states of the former Soviet Union
    Chhabra, P; Samoilovich, E; Yermalovich, M; Chernyshova, L; Gheorghita, S; Cojocaru, R; Shugayev, N; Sahakyan, G; Lashkarashvili, M; Chubinidze, M; Zakhashvili, K; Videbaek, D; Wasley, A; Vinjé, J
  • Epidemiological study of human calicivirus infection in children with gastroenteritis in Lanzhou from 2001 to 2007
    Cheng, WX; Ye, XH; Yang, XM; Li, YN; Jin, M; Jin, Y; Duan, ZJ
  • Molecular detection of genogroup I sapovirus in Tunisian children suffering from acute gastroenteritis
    Sdiri-Loulizi, K; Hassine, M; Gharbi-Khelifi, H; Aouni, Z; Chouchane, S; Sakly, N; Neji-Guédiche, M; Pothier, P; Ambert-Balay, K; Aouni, M
  • Molecular epidemiology and genetic diversity of human astrovirus in South Korea from 2002 to 2007
    Jeong, AY; Jeong, HS; Jo, MY; Jung, SY; Lee, MS; Lee, JS; Jee, YM; Kim, JH; Cheon, DS

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