Arch Virol (2001) 146: 1391–1397
Molecular characterization and expression of the M6 gene
of grass carp hemorrhage virus (GCHV), an aquareovirus
T. Qiu, R. H. Lu, J. Zhang, and Z. Y. Zhu
State Key Laboratory of Freshwater Ecology and Biotechnology,
Institute of Hydrobiology, Chinese Academy of Sciences,
Accepted February 28, 2001
Summary. The complete nucleotide sequence of M6 gene of grass carp hemor-
rhage virus (GCHV) was determined. It is 2039 nucleotides in length and contains
a single large open reading frame that could encode a protein of 648 amino acids
with predicted molecular mass of 68.7 kDa. Amino acid sequence comparison
revealed that the protein encoded by GCHV M6 is closely related to the protein
l of mammalian reovirus. The M6 gene, encoding the major outer-capsid pro-
tein, was expressed using the pET fusion protein vector in Escherichia coli and
detected by Western blotting using chicken anti-GCHV immunoglobulin (IgY).
The result indicates that the protein encoded by M6 may share a putative Asn-
42-Pro-43 proteolytic cleavage site with l.
Aquareovirus is an important pathogen involved in diseases of aquatic animals.
It is a genus in the Reoviridae family. The virions consist of a double-layered
capsid containing a genome composed of 11 segments of double-stranded RNA.
A large number of aquareoviruses have been isolated from different ﬁsh, shellﬁsh
and crustacean species. Not all these aquareoviruses have been associated with
clinical disease. Many viruses were isolated from normal ﬁsh . Grass carp
hemorrhage virus (GCHV) , as a member of aquareovirus, is a pathogen caus-
ing hemorrhagic disease of grass carp, and was found to be the most pathogenic
aquareovirus . Thus it has obtained more attention. GCHV shares the com-
mon physicochemical properties and morphological characteristics with other
The nucleotide sequence data for GCHV M6 reported in this paper can be accessed in
the GenBank databases under the accession number AF239175.