Molecular characterization and expression analysis of the gene for zeaxanthin epoxidase from ‘Yinhong’ grape (Vitis vinifera L.)

Molecular characterization and expression analysis of the gene for zeaxanthin epoxidase from... The dual-function monooxygenase zeaxanthin epoxidase (ZEP) is embedded in the thylakoid membrane and positioned on one side of the thylakoid membrane matrix. In this study, ZEP cDNA was cloned and the specific expression vector PBI121-VvZEP was constructed to study the enzymatic control of abscisic acid (ABA) synthesis. We hypothesized that the VvZEP genes can be transferred into Arabidopsis thaliana (L.) Heynh plants via the Agrobacterium-mediated impregnation method. ZEP was cloned from the ‘Yinhong’ grape (Vitis vinifera L.) cultivar and designated as VvZEP. Quantitative real-time polymerase chain reaction analyses showed that grape accumulated higher levels of VvZEP mRNA during the later stages of development than during early stages. VvZEP transcripts were detected in all parts of the analyzed grape plant, although the highest levels were found in the fruits. Gas chromatography analysis of ABA showed the highest ABA concentration in ripe fruits. Transgenic analysis revealed that VvZEP was successfully transformed into A. thaliana and showed no obvious effects on seed germination. The flowering stage and time of seed maturity were significantly increased in transgenic Arabidopsis. Our results will help elucidate the effects of overexpression of the VvZEP gene on the ripening stage of grape. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Brazilian Journal of Botany Springer Journals

Molecular characterization and expression analysis of the gene for zeaxanthin epoxidase from ‘Yinhong’ grape (Vitis vinifera L.)

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Publisher
Springer International Publishing
Copyright
Copyright © 2018 by Botanical Society of Sao Paulo
Subject
Life Sciences; Plant Systematics/Taxonomy/Biogeography
ISSN
0100-8404
eISSN
1806-9959
D.O.I.
10.1007/s40415-018-0451-2
Publisher site
See Article on Publisher Site

Abstract

The dual-function monooxygenase zeaxanthin epoxidase (ZEP) is embedded in the thylakoid membrane and positioned on one side of the thylakoid membrane matrix. In this study, ZEP cDNA was cloned and the specific expression vector PBI121-VvZEP was constructed to study the enzymatic control of abscisic acid (ABA) synthesis. We hypothesized that the VvZEP genes can be transferred into Arabidopsis thaliana (L.) Heynh plants via the Agrobacterium-mediated impregnation method. ZEP was cloned from the ‘Yinhong’ grape (Vitis vinifera L.) cultivar and designated as VvZEP. Quantitative real-time polymerase chain reaction analyses showed that grape accumulated higher levels of VvZEP mRNA during the later stages of development than during early stages. VvZEP transcripts were detected in all parts of the analyzed grape plant, although the highest levels were found in the fruits. Gas chromatography analysis of ABA showed the highest ABA concentration in ripe fruits. Transgenic analysis revealed that VvZEP was successfully transformed into A. thaliana and showed no obvious effects on seed germination. The flowering stage and time of seed maturity were significantly increased in transgenic Arabidopsis. Our results will help elucidate the effects of overexpression of the VvZEP gene on the ripening stage of grape.

Journal

Brazilian Journal of BotanySpringer Journals

Published: Jun 6, 2018

References

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