Molecular characterisation of plant parasitic nematode
Longidorus poessneckensis Altherr, 1974 (Nematoda:
Longidoridae)
Franciszek Wojciech Kornobis
&
Sergei A. Subbotin
&
Shesh Kumari
Accepted: 27 December 2017 /Published online: 13 January 2018
#
Koninklijke Nederlandse Planteziektenkundige Vereniging 2018
Abstract The plant parasitic nematode Longidorus
poessneckensis found in Austria, the Czech Republic,
Germany, Poland and the Slovak Republic was molec-
ularly characterized. Mitochondrial genes encoding cy-
tochrome c oxidase subunit I (COI) and nicotinamide
dehydrogenase subunit 4 (nad4), the D2 and D3 ex-
pansion segments of 28S rRNA and Internal tran-
scribed spacer 1 (ITS1) rRNA were sequenced for
16 L. poessneckensis populations. Six haplotypes of
COI and five haplotypes of nad4 were determined.
Nucleotide intraspecific variation was up to 17.1%
for the partial sequenced COI gene and up to 17.7%
for the partial sequenced nad4 gene, the latter being
the highest up to date known intraspecific variation in
this genus. The analyses of multiple amino acid se-
quence alignments of mitochondrial genes revealed
low variability (0–2.4%) for COI gene and high diver-
gence (0–7.6%) for nad4 gene. Intraspecific sequence
diversity for the D2-D3 of 28S rRNA gene was up to
1.2% and for ITS1 rRNA gene was up to 1.6%. It has
been hypothesized, that during the Last Glacial Max-
imum, L. poessneckensis populations probably
persisted in refuge areas in the Carpathian Mountains
and subsequently expanding from these areas and col-
onizing other European regions.
Keywords COI gene
.
Mitochondrial DNA
.
nad4gene
.
Nematode
.
Ribosomal RNA
Introduction
Plant parasitic nematodes of genus Longidorus
(Nematoda, Dorylaimida, Longidoridae) are po-
lyphagous root-ectoparasites. They are polypha-
gous root-ectoparasites and cause damage to a
wide range of plants. Some of the species are of
economic importance as pests of agricultural crops.
They cause damage to plant either by feeding on
roots or by the ability of some species to transmit
plant nepoviruses (Taylor and Brown 1997).
The genetic characterization of plant parasitic
nematodes is important for diagnosis, studying
population biology and genetic structure of nema-
todes. Molecular approaches have been used to
characterize and explore the population genetic
Eur J Plant Pathol (2018) 151:791–802
https://doi.org/10.1007/s10658-017-1417-0
F. W. Kornobis
Department of Zoology, Institute of Plant Protection-National
Research Institute, Władysława Węgorka 20, 60-318 Poznań,
Poland
S. A. Subbotin
Plant Pest Diagnostic Center, California Department of Food and
Agriculture, 3294 Meadowview Road, Sacramento, CA
95832-1448, USA
S. A. Subbotin
Center of Parasitology, A.N. Severtsov Institute of Ecology and
Evolution of the Russian Academy of Sciences, Leninskii
Prospect 33, Moscow 117071, Russia
S. Kumari (*)
Division of Crop Protection and Plant Health, Crop Research
Institute, Drnovská 507, Ruzyně, 16106 Prague 6, Czech Republic
e-mail: kumari@vurv.cz
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