Molecular characterisation of plant parasitic nematode Longidorus poessneckensis Altherr, 1974 (Nematoda: Longidoridae)

Molecular characterisation of plant parasitic nematode Longidorus poessneckensis Altherr, 1974... The plant parasitic nematode Longidorus poessneckensis found in Austria, the Czech Republic, Germany, Poland and the Slovak Republic was molecularly characterized. Mitochondrial genes encoding cytochrome c oxidase subunit I (COI) and nicotinamide dehydrogenase subunit 4 (nad4), the D2 and D3 expansion segments of 28S rRNA and Internal transcribed spacer 1 (ITS1) rRNA were sequenced for 16 L. poessneckensis populations. Six haplotypes of COI and five haplotypes of nad4 were determined. Nucleotide intraspecific variation was up to 17.1% for the partial sequenced COI gene and up to 17.7% for the partial sequenced nad4 gene, the latter being the highest up to date known intraspecific variation in this genus. The analyses of multiple amino acid sequence alignments of mitochondrial genes revealed low variability (0–2.4%) for COI gene and high divergence (0–7.6%) for nad4 gene. Intraspecific sequence diversity for the D2-D3 of 28S rRNA gene was up to 1.2% and for ITS1 rRNA gene was up to 1.6%. It has been hypothesized, that during the Last Glacial Maximum, L. poessneckensis populations probably persisted in refuge areas in the Carpathian Mountains and subsequently expanding from these areas and colonizing other European regions. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Plant Pathology Springer Journals

Molecular characterisation of plant parasitic nematode Longidorus poessneckensis Altherr, 1974 (Nematoda: Longidoridae)

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Publisher
Springer Journals
Copyright
Copyright © 2018 by Koninklijke Nederlandse Planteziektenkundige Vereniging
Subject
Life Sciences; Plant Pathology; Plant Sciences; Ecology; Agriculture; Life Sciences, general
ISSN
0929-1873
eISSN
1573-8469
DOI
10.1007/s10658-017-1417-0
Publisher site
See Article on Publisher Site

Abstract

The plant parasitic nematode Longidorus poessneckensis found in Austria, the Czech Republic, Germany, Poland and the Slovak Republic was molecularly characterized. Mitochondrial genes encoding cytochrome c oxidase subunit I (COI) and nicotinamide dehydrogenase subunit 4 (nad4), the D2 and D3 expansion segments of 28S rRNA and Internal transcribed spacer 1 (ITS1) rRNA were sequenced for 16 L. poessneckensis populations. Six haplotypes of COI and five haplotypes of nad4 were determined. Nucleotide intraspecific variation was up to 17.1% for the partial sequenced COI gene and up to 17.7% for the partial sequenced nad4 gene, the latter being the highest up to date known intraspecific variation in this genus. The analyses of multiple amino acid sequence alignments of mitochondrial genes revealed low variability (0–2.4%) for COI gene and high divergence (0–7.6%) for nad4 gene. Intraspecific sequence diversity for the D2-D3 of 28S rRNA gene was up to 1.2% and for ITS1 rRNA gene was up to 1.6%. It has been hypothesized, that during the Last Glacial Maximum, L. poessneckensis populations probably persisted in refuge areas in the Carpathian Mountains and subsequently expanding from these areas and colonizing other European regions.

Journal

European Journal of Plant Pathology – Springer Journals

Published: Jan 13, 2018

References

  • Nématodes de la nappe phréatique du réseau fluvial de la Saale (Thuringe) II
    Altherr, E
  • Unravelling the biodiversity and molecular phylogeny of needle nematodes of the genus Longidorus (Nematoda: Longidoridae) in olive and a description of six new species
    Archidona-Yuste, A; Navas-Cortés, JA; Cantalapiedra-Navarrete, C; Palomares-Rius, JE; Castillo, P
  • Median-joining networks for inferring intraspecific phylogenies
    Bandelt, HJ; Forster, P; Röhl, A
  • Substitution bias, rapid saturation, and the use of mtDNA for nematode systematics
    Blouin, MS; Yowell, CA; Courtney, CH; Dame, JB
  • The internal transcribed spacer region of Belonolaimus (Nemata, Belonolaimidae)
    Cherry, T; Szalanski, AL; Todd, TC; Powers, TO
  • Morphological and molecular characterisation of the Saratov population of the European dagger nematode, Xiphinema diversicaudatum (Nematoda: Dorylaimida), with notes on phylogeography of the species
    Chizhov, VN; Pridannikov, MV; Peneva, V; Subbotin, SA
  • Linking DNA sequences to morphology: Cryptic diversity and population genetic structure in the marine nematode Thoracostoma trachygaster (Nematoda, Leptosomatidae)
    Derycke, S; Ley, P; Ley, IT; Holovachov, O; Rigaux, A; Moens, T
  • Genetic structure of Xiphinema pachtaicum and X. index populations based on mitochondrial DNA variation
    Gutiérrez-Gutiérrez, C; Castillo, P; Cantalapiedra-Navarrete, C; Landa, BB; Derycke, S; Palomares-Rius, JE
  • A molecular phylogenetic approach to Longidoridae (Nematoda: Dorylaimida)
    He, Y; Subbotin, SA; Rubtsova, TV; Lamberti, F; Brown, DJF; Moens, M
  • The mitochondrial genome of Xiphinema americanum sensu stricto (Nematoda: Enoplea): Considerable economization in the length and structural features of encoded genes
    He, Y; Jones, J; Armstrong, M; Lamberti, F; Moens, M
  • MrBAYES: Bayesian inference of phylogenetic trees
    Huelsenbeck, JP; Ronquist, F
  • Toward next-generation sequencing of mitochondrial genomes- focus on parasitic worms of animals and biotechnological implications
    Jex, AR; Littlewood, DTJ; Gasser, RB
  • Longidorus poessneckensis Altherr, 1974 and L. piceicola Liskova, Robbins & Brown, 1997 (Nematoda: Longidoridae): New records from Poland and the first description of the L. poessneckensis male and a bivulval female
    Kornobis, FW; Peneva, V
  • Characterization of Longidorus caespiticola (Nematoda: Longidoridae) from the Czech Republic
    Kumari, S
  • Characterization of Longidorus helveticus (Nematoda: Longidoridae) from the Czech Republic
    Kumari, S; Subbotin, SA
  • Molecular and morphological delineation of Longidorus poessneckensis Altherr, 1974 (Nematoda: Dorylaimida)
    Kumari, S; Decraemer, W; Traversa, D; Lišková, M
  • Ribosomal and mitochondrial DNA analyses of Xiphinema americanum-group populations
    Lazarova, SS; Malloch, G; Oliveira, CMG; Hübschen, J; Neilson, R
  • Occurrence and ecology of Longidoridae (Nematoda: Dorylaimida) in floodplain forests in the Slovak Republic
    Lišková, M; Sturhan, D
  • Nematode molecular evolution: An investigation of evolutionary patterns among nematodes based upon DNA sequences
    Nunn, GB
  • Mitochondrial genome diversity in dagger and needle nematodes (Nematoda: Longidoridae)
    Palomares-Rius, JE; Cantalapiedra-Navarrete, C; Archidona-Yuste, A; Blok, VC; Castillo, P
  • The utility of mtDNA and rDNA for barcoding and phylogeny of plant-parasitic nematodes from Longidoridae (Nematoda, Enoplea)
    Palomares-Rius, JE; Cantalapiedra-Navarrete, C; Archidona-Yuste, A; Subbotin, SA; Castillo, P
  • Molecular biogeography of Europe: Pleistocene cycles and postglacial trends
    Schmitt, T
  • Non–manual lysis of second–stage Meloidogyne juveniles for identification of pure and mixed samples based on the polymerase chain reaction
    Stanton, JM; McNicol, CD; Steele, V
  • Redescription of Longidorus poessneckensis Altherr, 1974 (Nematoda: Dorylaimida)
    Sturhan, D; Loof, PAA
  • Molecular characterisation and diagnostics of some Longidorus species (Nematoda: Longidoridae) from Russia and other countries using rRNA genes
    Subbotin, SA; Rogozhin, EA; Chizhov, VN
  • Characterisation of populations of Longidorus orientalis Loof, 1982 (Nematoda: Dorylaimida) from date palm (Phoenix dactylifera L.) in the USA and other countries and incongruence of phylogenies inferred from ITS1 rRNA and coxI genes
    Subbotin, SA; Stanley, JD; Ploeg, AT; Maafi, ZT; Tzortzakakis, EA; Chitambar, JJ; Palomares-Rius, JE; Castillo, P; Inserty, RN
  • PAUP*: Phylogenetic analysis using parsimony (*and other methods), version 4.0b 10. Sunderland, MA
    Swofford, DL
  • Nematode vectors of plant viruses
    Taylor, CE; Brown, DJF
  • The CLUSTAL_X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools
    Thompson, JD; Gibbon, TJ; Plewniak, F; Jeanmougin, F; Higgins, DG
  • Phylogenetic relationships and genetic variation in Longidorus and Xiphinema species (Nematoda: Longidoridae) using ITS1 sequences of nuclear ribosomal DNA
    Ye, W; Szalanski, AL; Robbins, RT

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