Arch Virol (2001) 146: 1821–1829
Molecular characterisation of an isolate of Dasheen mosaic virus
from Zantedeschia aethiopica in China and comparisons
in the genus Potyvirus
, Jianping Chen
, Jishuang Chen
, and M. J. Adams
College of Life Science, Zhejiang University, Hangzhou, China
Virology Laboratory, Zhejiang Academy of Agricultural Sciences, Hangzhou, China
Plant Pathology Department, IACR-Rothamsted, Harpenden, U.K.
Accepted April 11, 2001
Summary. The complete nucleotide sequence of an isolate of Dasheen mosaic
virus from ZantedeschiaaethiopicainZhejiangProvince,China, was determined.
The 9991 nucleotide genome was typical of the genus Potyvirus and phylogenetic
analysis showed it to be a member of the Bean common mosaic virus subgroup.
-terminal sequence, including the coat protein region, was determined for
three further isolates from China and Japan. Variations in the length and compo-
sition of the N-terminus of the coat protein were not related to geographic origin
or plant host. An analysis of all potyvirus cleavage sites revealed patterns related
to phylogenetic groupings.
Dasheen mosaic virus (DsMV) is the most important viral pathogen of culti-
vated aroid plants worldwide, including the genera Aglaonema, Alocasia,
Amorphophallus, Anthurium, Arisaema, Caladium, Colocasia, Cryptocoryne,
Dieffenbachia, Monstera, Philodendron, Richardia, Spathiphyllum, Xanthosoma
and Zantedeschia. DsMV is a typical member of the genus Potyvirus, with
ﬂexuous, ﬁlamentous particles about 750 nm in length containing a positivesense,
single-stranded RNA genome [1, 8, 10]. The partial nucleotide sequences of four
isolates of the virus from a variety of sources have been reported [4–7]. Zant-
edeschia aethiopica is an ornamental plant commonly exchanged between coun-
tries and continents, but its diseases have been little studied. We have found plants
of this species severely infected by DsMV and now report the characterisation of
one isolate (DsMV-M13), and the complete nucleotide sequence of its genome.