A novel cypovirus, assigned CoCPV, was isolated from natural populations of the western spruce budworm, Choristoneura occidentalis . The complete nucleotide sequences of genomic segments S2–S5 and S7–S10 were determined. Each segment contained a single open reading frame. Conserved motifs 5′ (AGUUU……UUUGUGC) 3′ were found at the ends of each segment. Analysis of S2, which encoded a putative RNA-dependent RNA polymerase protein, confirmed CoCPV belonged to the genus Cypovirus within the family Reoviridae . Further phylogenetic analysis using S10 (the polyhedrin gene) aligned this virus with species type-16, closely related to a cypovirus isolated from C. fumiferana .
Archives of Virology – Springer Journals
Published: Sep 1, 2008
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