Arch Virol (2002) 147: 411–417
Molecular characterisation and evolutionary relationships
of a potyvirus infecting Crotalaria in Brazil
D. S. Freitas
, I. G. Maia
, P. Arruda
, and J. Vega
Departamento de Fisiologia Vegetal, Universidade Estadual de Campinas,
Campinas, SP, Brazil
Centro de Biologia Molecular e Engenharia Genética,
Universidade Estadual de Campinas, Campinas, SP, Brazil
Accepted October 5, 2001
Summary. The 3
terminal genomic region of a potyvirus causing mosaic disease
in several Crotalaria species has been cloned and sequenced. Comparisons of
the nucleotide and deduced amino acid (aa) sequences of the cloned cDNA with
those from other potyviruses show that the Crotalaria-infectingvirus (designated
Crotalaria mosaic virus; CrMV) is closely related to Cowpea aphid-borne mosaic
virus (CABMV). Maximum identity (95.4%) at the coat protein (CP) aa level
was observed between CrMV and a Brazilian strain of CABMV. Phylogenetic
analyses derived from the sequence alignments of the CP and 3
region conﬁrmed the identiﬁcation of CrMV as a strain of CABMV and the name
CABMV-Cr is suggested.
An aphid-transmitted virus was found to be the causal agent of a mosaic disease
of Crotalaria species in the state of São Paulo, Brazil. The virus, designated
Crotalaria mosaic virus (CrMV), was tentatively classiﬁed as a member of the
Potyvirus genus on the basis of biological properties and particle morphology .
CrMV is nonpersistently transmissible by Myzus persicae, M. nicotianae, and
Aphis gossypii . It was found to be serologically related to a potyvirus causing
The nucleotide sequence data reported in this paper has been assigned GeneBank
accession number AF368424.
Present address: Instituto de Biociências, Departamento de Gen´etica, Universidade
Estadual Paulista, 18618-000, Botucatu, SP, Brazil.