Molecular and physiological responses of Arabidopsis thaliana plants deficient in the genes responsible for ABA and cytokinin reception and metabolism to heat shock

Molecular and physiological responses of Arabidopsis thaliana plants deficient in the genes... Using mutant plants of Arabidopsis thaliana, participation of the genes involved in abscisic acid (ABA) and cytokinins (CKs) metabolism and signaling in plant defense responses to heat shock (HS) was investigated. The magnitude of the stress action was assessed with biochemical indicators, such as accumulation of proline and malonic dialdehyde (MDA) and changes in the content of gene transcripts of heat shock proteins (HSPs) (HSP90.1 and HSP90.5) and transcription factor of HS (HSFA2) as well as stress-inducible genes, markers of oxidative stress (AOX1a, RD29, PRODH1, and P5CS1). Mutants with inactivated genes of ABA synthesis and, especially, signal perception exhibited lower thermo-resistance and accumulated elevated amounts of CK metabolism and signal transduction genes. In contrast, plants with inactivated components of CK synthesis and signal perception displayed increased tolerance to high temperatures and reduced levels of mRNA of oxidative stress genes as compared to wild type (WT) plants. However, enhancement of HS contributed to decrease of thermo-resistance of CKs receptor mutants up to the level of WT plants. Under hyperthermia, the stimulation of the plant defense mechanisms was accompanied by downregulation of the expression of CK metabolism and signal transduction genes (IPT3, CKX1, ARR5, AHK2, and AHK4) and ABA catabolism gene (CYP707A1) and upregulation of the expression of ABA synthesis and signal perception genes (ABA3 and ABI2). In the mutants insensitive to ABA, CYP707A1 gene was upregulated under the HS, while the expression of CK receptor gene expression did not reliably change. The results indicate that the response of the plants to elevated temperatures was determined not only by strength and the duration of the stress but the state of their ABA and CK metabolic and signaling systems as well. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Molecular and physiological responses of Arabidopsis thaliana plants deficient in the genes responsible for ABA and cytokinin reception and metabolism to heat shock

Loading next page...
 
/lp/springer_journal/molecular-and-physiological-responses-of-arabidopsis-thaliana-plants-nF69TB0uFS
Publisher
Pleiades Publishing
Copyright
Copyright © 2016 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443716030043
Publisher site
See Article on Publisher Site

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial