Arch Virol (1998) 143: 1021–1028
Molecular and immunologic characterization of group A bovine
rotavirus ﬁeld isolates with P8 spike protein
R. E. Campbell, W. Lu
, A. K. White, and G. E. Duhamel
Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln,
Lincoln, Nebraska, U.S.A.
Accepted December 15, 1997
Summary. Bovine rotavirus strain B223 is the North American prototype for
group A P8 serotype/genotype rotaviruses. Rotaviruses with this serotype/
genotypeareaprevalentcauseofneonatalcalfdiarrheaandhave also been isolated
from asymptomatic human infants. On the basis of deduced amino acid sequence
of the outer capsid viral protein 4 (VP4), strain B223 lacks a cysteine at position
318 that is conserved among all other rotavirus strains. It has been speculated
that this may result in the loss of disulﬁde bond and a change in the structure of
the VP4 that may affect the infectivity and antigenicity of the virus. This paper
describes partial sequences of the VP4 gene (nucleotides 613 to 1 016 coding for
amino acid positions 202 to 335) of 16 bovine rotavirus ﬁeld isolates with P8
that were obtained from calves in Nebraska and Indiana. All the isolates lack a
cystein at position 203 and had a conserved valine residue at position 318, thus
indicating that the prototype strain B223 is representative of group A rotaviruses
with P8 serotype/genotype.
Rotaviruses are a major cause of viral enteritis in human infants as well as many
animals species . Signiﬁcant mortality occurs in infants in developing nations,
but morbidity due to rotavirus infection is found worldwide . Although a
commercial vaccine has been available for over 25 years, rotavirus-associated
This paper represents a portion of a thesis submitted by R. E. Campbell to the University
of Nebraska-Lincoln in partial fulﬁllment of the requirements for the MS degree.
Present address: Ambico, Inc., Dallas Center, Iowa, U.S.A.