Mammalian Genome 11, 238–240 (2000). Incorporating Mouse Genome © Springer-Verlag New York Inc. 2000 Molecular and embryological characterization of a new transgene-induced null allele of mouse Brachyury locus 1 1,2 2 Kuniya Abe, Ken-ichi Yamamura, Misao Suzuki Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, 4-24-1 Kuhonji, Kumamoto 862-0976, Japan Center for Animal Resources and Research Development, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan Received: 13 July 1999 / Accepted: 20 September 1999 During establishment of transgenic mouse lines, transgene inser- region in wild-type mouse, we found that the pattern was different tion into genome may result in disruption of endogenous gene. in the left half of the insert (Fig. 1), suggesting the presence of a Such a serendipity often discloses normal function of an endog- junction in the middle of the insert. We then subcloned a BglII- enous gene. We have made several transgenic lines carrying rat KpnI fragment that might contain the integration junction se- c-myc gene driven by human metallothionein (MT) promoter. The quence. DNA sequence analysis of the fragment showed that one expression of the transgene leads to male sterility caused by apop- end of the insert contained a part of the
Mammalian Genome – Springer Journals
Published: Feb 26, 2014
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