Molecular and biological properties of tomato necrotic stunt virus and development of a sensitive real-time RT-PCR assay

Molecular and biological properties of tomato necrotic stunt virus and development of a sensitive... The newly identified tomato necrotic stunt virus (ToNStV), with a genome of 10,057 nucleotides, had 60 % nucleotide sequence and 70 % coat protein amino acid sequence identity to other potyviruses. Analysis of a second ToNStV isolate revealed two single nucleotide substitutions. ToNStV induced stunting and chlorotic to necrotic leaf symptoms on tomato plants. In host-range assays, ToNStV systemically infected many species in the family Solanaceae and locally infected some species in the families Amaranthaceae, Brassicaceae and Cucurbitaceae . A sensitive and reliable real-time RT-PCR assay was developed using simply prepared crude tissue extract. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Molecular and biological properties of tomato necrotic stunt virus and development of a sensitive real-time RT-PCR assay

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Publisher
Springer Journals
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1830-4
Publisher site
See Article on Publisher Site

Abstract

The newly identified tomato necrotic stunt virus (ToNStV), with a genome of 10,057 nucleotides, had 60 % nucleotide sequence and 70 % coat protein amino acid sequence identity to other potyviruses. Analysis of a second ToNStV isolate revealed two single nucleotide substitutions. ToNStV induced stunting and chlorotic to necrotic leaf symptoms on tomato plants. In host-range assays, ToNStV systemically infected many species in the family Solanaceae and locally infected some species in the families Amaranthaceae, Brassicaceae and Cucurbitaceae . A sensitive and reliable real-time RT-PCR assay was developed using simply prepared crude tissue extract.

Journal

Archives of VirologySpringer Journals

Published: Feb 1, 2014

References

  • Overview and analysis of the polyprotein cleavage sites in the family Potyviridae
    Adams, MJ; Antoniw, JF; Beaudoin, F
  • Molecular criteria for genus and species discrimination within the family Potyviridae
    Adams, MJ; Antoniw, JF; Fauquet, CM

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