Molecular analysis of a novel species, Gangesia punjabensis (Family: Proteocephalidae, Subfamily: Gangesiinae) infecting an Indian freshwater cat fish, Wallago attu evidencing species complex

Molecular analysis of a novel species, Gangesia punjabensis (Family: Proteocephalidae, Subfamily:... In the present study, from August 2015 to July 2016, a total of 159 specimens of Wallago attu Bloch and Scheider, 1801 vern. mully were collected from the Ropar wetland, Punjab, India and were examined for the presence of intestinal tapeworms. The infection rate was 12.5%, out of which, two fishes were heavily infected with a proteocephalidean cestode belonging to the genus Gangesia in the month of February. Gangesia punjabensis sp. nov. was identified and described on the basis of morphology and partial amplification of LSUrDNA (28S rDNA). The histopathogenesis caused by these parasites have also been studied in histological sections. About 9–35 number of worms were collected from a single host. The length of worms ranged from 6.0 to 6.2 cm. The strobila consisted of an armed scolex, neck, immature proglottids (approximately 90 in number) and mature proglottids (approximately 7 in number) and gravid proglottids were not detected. Analysis of 28S large subunit (LSU) rDNA sequence of the isolate of G. punjabensis n. sp. demonstrated maximum similarity of 99% with G. bengalensis, 98% with G. vachai, 97% with G. macrones. The phylogenetic tree based on the final edited alignment (822 bp) with Neighbour-Joining showed the highest bootstrap value of 100 and placed G. punjabensis n. sp. with G. bengalensis and G. macrones in the same clade showing them to be a species complex. The intestinal mucosal lining exhibited blister formation, haemorrhagia and inflammation due to the presence of deeply embedded scolices. Histopathology caused was evident due to disruption of villous surface and cellular infiltration. This is the first report of cestodes belonging to the genus Gangesia from Ropar wetland, Punjab, India. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Parasitic Diseases Springer Journals

Molecular analysis of a novel species, Gangesia punjabensis (Family: Proteocephalidae, Subfamily: Gangesiinae) infecting an Indian freshwater cat fish, Wallago attu evidencing species complex

Loading next page...
 
/lp/springer_journal/molecular-analysis-of-a-novel-species-gangesia-punjabensis-family-F5I41o7krG
Publisher
Springer India
Copyright
Copyright © 2017 by Indian Society for Parasitology
Subject
Medicine & Public Health; Infectious Diseases; Health Promotion and Disease Prevention; Medicine/Public Health, general
ISSN
0971-7196
eISSN
0975-0703
D.O.I.
10.1007/s12639-017-0912-8
Publisher site
See Article on Publisher Site

Abstract

In the present study, from August 2015 to July 2016, a total of 159 specimens of Wallago attu Bloch and Scheider, 1801 vern. mully were collected from the Ropar wetland, Punjab, India and were examined for the presence of intestinal tapeworms. The infection rate was 12.5%, out of which, two fishes were heavily infected with a proteocephalidean cestode belonging to the genus Gangesia in the month of February. Gangesia punjabensis sp. nov. was identified and described on the basis of morphology and partial amplification of LSUrDNA (28S rDNA). The histopathogenesis caused by these parasites have also been studied in histological sections. About 9–35 number of worms were collected from a single host. The length of worms ranged from 6.0 to 6.2 cm. The strobila consisted of an armed scolex, neck, immature proglottids (approximately 90 in number) and mature proglottids (approximately 7 in number) and gravid proglottids were not detected. Analysis of 28S large subunit (LSU) rDNA sequence of the isolate of G. punjabensis n. sp. demonstrated maximum similarity of 99% with G. bengalensis, 98% with G. vachai, 97% with G. macrones. The phylogenetic tree based on the final edited alignment (822 bp) with Neighbour-Joining showed the highest bootstrap value of 100 and placed G. punjabensis n. sp. with G. bengalensis and G. macrones in the same clade showing them to be a species complex. The intestinal mucosal lining exhibited blister formation, haemorrhagia and inflammation due to the presence of deeply embedded scolices. Histopathology caused was evident due to disruption of villous surface and cellular infiltration. This is the first report of cestodes belonging to the genus Gangesia from Ropar wetland, Punjab, India.

Journal

Journal of Parasitic DiseasesSpringer Journals

Published: Mar 29, 2017

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from
Google Scholar,
PubMed
Create lists to
organize your research
Export lists, citations
Read DeepDyve articles
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off