Modulation of Voltage-Dependent K+ Channel Current in Vascular Smooth Muscle Cells from Rat Mesenteric Arteries

Modulation of Voltage-Dependent K+ Channel Current in Vascular Smooth Muscle Cells from Rat... Voltage-dependent K+ (Kv) channels were studied in smooth muscle cells (SMCs) freshly isolated from rat mesenteric arteries. A delayed outward rectifier Kv current (I K) with a weak voltage dependence was identified. The amplitude of I K, but not its inactivation kinetics, was inhibited by 4-aminopyridine (4-AP) (IC50, 5.1 ± 0.9 mm). The inhibitory effect of 4-AP was not use-dependent, and the unbinding of 4-AP from I K channels was complete in the absence of depolarization stimuli, suggesting the binding of 4-AP to the closed state of Kv channels. There was no change in the steady-state inactivation, but the steady-state activation curve of I K was shifted in the presence of 4-AP by +6 mV. Including 4-AP in pipette solution instantly inhibited I K upon the rupture of cell membrane, indicating that 4-AP bound to the inner mouth of Kv channel pores. Several Kv channel proteins encoding the native I K-type Kv channels, but not the transient outward A-type Kv channels, were identified. Among the identified I K-encoding gene transcripts, the expression of Kv1.5 was the most abundant. Our results elucidate the modulating mechanisms for the 4-AP-induced I K inhibition in rat mesenteric artery SMCs and suggest that the unique properties of Kv channels in these cells might be related to the heteromeric expression of the I K-encoding genes with Kv1.5 subunit playing an important role. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Modulation of Voltage-Dependent K+ Channel Current in Vascular Smooth Muscle Cells from Rat Mesenteric Arteries

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Publisher
Springer-Verlag
Copyright
Copyright © Inc. by 2001 Springer-Verlag New York
Subject
Life Sciences; Biochemistry, general; Human Physiology
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s002320010067
Publisher site
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