Thin layer chromatography is an important tool for detecting amino acids by several spray reagents. Two new spray reagents (picric acid and o-chloranil) with high sensitivities for easy and rapid identification of amino acids on thin-layer chromatography plates have been introduced. The reagents are capable of developing various distinguishable colors with many of them. The color pictures of the chromatograms by digital camera are also included to demonstrate the different colors. The detection limits for picric acid and o-chloranil are 0.04–0.80 and 0.10–8.00 μg, respectively. A probable mechanism for such color formation has also been proposed.
Research on Chemical Intermediates – Springer Journals
Published: Sep 14, 2013
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