Modified ninhydrin reagents to detect amino acids on TLC plates

Modified ninhydrin reagents to detect amino acids on TLC plates Thin layer chromatography is an important tool for detecting amino acids by several spray reagents. Two new spray reagents (picric acid and o-chloranil) with high sensitivities for easy and rapid identification of amino acids on thin-layer chromatography plates have been introduced. The reagents are capable of developing various distinguishable colors with many of them. The color pictures of the chromatograms by digital camera are also included to demonstrate the different colors. The detection limits for picric acid and o-chloranil are 0.04–0.80 and 0.10–8.00 μg, respectively. A probable mechanism for such color formation has also been proposed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Research on Chemical Intermediates Springer Journals

Modified ninhydrin reagents to detect amino acids on TLC plates

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Publisher
Springer Netherlands
Copyright
Copyright © 2013 by Springer Science+Business Media Dordrecht
Subject
Chemistry; Catalysis; Physical Chemistry; Inorganic Chemistry
ISSN
0922-6168
eISSN
1568-5675
D.O.I.
10.1007/s11164-013-1388-5
Publisher site
See Article on Publisher Site

Abstract

Thin layer chromatography is an important tool for detecting amino acids by several spray reagents. Two new spray reagents (picric acid and o-chloranil) with high sensitivities for easy and rapid identification of amino acids on thin-layer chromatography plates have been introduced. The reagents are capable of developing various distinguishable colors with many of them. The color pictures of the chromatograms by digital camera are also included to demonstrate the different colors. The detection limits for picric acid and o-chloranil are 0.04–0.80 and 0.10–8.00 μg, respectively. A probable mechanism for such color formation has also been proposed.

Journal

Research on Chemical IntermediatesSpringer Journals

Published: Sep 14, 2013

References

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