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P. Sharma, A. Lönneborg (1996)
Isolation and characterization of a cDNA encoding a plant defensin-like protein from roots of Norway sprucePlant Molecular Biology, 31
C. Merril, M. Dunau, D. Goldman (1981)
A rapid sensitive silver stain for polypeptides in polyacrylamide gels.Analytical biochemistry, 110 1
K. Thevissen, B. Cammue, K. Lemaire, J. Winderickx, R. Dickson, R. Lester, Kathelijne Ferket, Frederic Even, A. Parret, W. Broekaert (2000)
A gene encoding a sphingolipid biosynthesis enzyme determines the sensitivity of Saccharomyces cerevisiae to an antifungal plant defensin from dahlia (Dahlia merckii).Proceedings of the National Academy of Sciences of the United States of America, 97 17
Ana Segura, M. Moreno, A. Molina, F. Garcı́a-Olmedo (1998)
Novel defensin subfamily from spinach (Spinacia oleracea)FEBS Letters, 435
(1996)
Isolation and Characterization of a cDNA Encoding a Plant DefensinLike Protein from Roots of Norway spruce , Plant
(2000)
Generation and Properties of Deletion Variants of Clostridium thermocellum Lichenase and Construction of Bifunctional Hybrid Proteins on Their Basis, Biokhimiya
W. Schaaper, G. Posthuma, H. Plasman, L. Sijtsma, F. Fant, F. Borremans, K. Thevissen, W. Broekaert, R. Meloen, A. Amerongen (2001)
Synthetic peptides derived from the beta2-beta3 loop of Raphanus sativus antifungal protein 2 that mimic the active site.The journal of peptide research : official journal of the American Peptide Society, 57 5
G. Samblanx, I. Goderis, K. Thevissen, R. Raemaekers, F. Fant, F. Borremans, D. Acland, R. Osborn, Sunil Patel, W. Broekaert (1997)
Mutational Analysis of a Plant Defensin from Radish (Raphanus sativus L.) Reveals Two Adjacent Sites Important for Antifungal Activity*The Journal of Biological Chemistry, 272
I. Kulaev (2004)
Several Words about Garri Izrailevich Abelev on His JubileeMolecular Biology, 37
R. Komakhin, I. Abdeeva, G. Dzhuzani, I. Goldenkova, A. Zhuchenko (2005)
Thermostable lichenase as a translational reporterRussian Journal of Genetics, 41
B. Janssen, H. Schirra, F. Lay, M. Anderson, D. Craik (2003)
Structure of Petunia hybrida defensin 1, a novel plant defensin with five disulfide bonds.Biochemistry, 42 27
I. François, M. Bolle, G. Dwyer, I. Goderis, Piet Woutors, P. Verhaert, P. Proost, W. Schaaper, B. Cammue, W. Broekaert (2002)
Transgenic Expression in Arabidopsis of a Polyprotein Construct Leading to Production of Two Different Antimicrobial Proteins1Plant Physiology, 128
I. Goldenkova, K. Musiychuk, E. Piruzian (2003)
Bifunctional Reporter Genes: Construction and Expression in Prokaryotic and Eukaryotic CellsMolecular Biology, 37
(1998)
Characterization of a Drought-Induced Soybean cDNA Encoding a Plant Defensin
U. Laemmli (1970)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 227
María Urdangarín, N. Norero, W. Broekaert, L. Canal (2000)
A defensin gene expressed in sunflower inflorescencePlant Physiology and Biochemistry, 38
B.P.H.J. Thomma, B.P.A. Cammue, K. Thevissen (2002)
Plant DefensinsPlanta, 216
F. Terras, K. Eggermont, Valentina Kovaleva, Natasha Raikhel, R. Osborn, Anthea Kester, Sarah Rees, Sophie Fred, Van Leuven, J. Vanderleyden, B. Cammue, Willem 'i (1995)
Small cysteine-rich antifungal proteins from radish: their role in host defense.The Plant cell, 7 5
W. Broekaert, F. Terras, B. Cammue, R. Osborn (1995)
Plant Defensins: Novel Antimicrobial Peptides as Components of the Host Defense System, 108
To achieve broader range of the defensin antimicrobial activity, based on the sd2 gene sequence, the modified gene, sd2mod, was constructed. Hybrid genes, sd2-licBM2, licBM2-sd2, licBM2-sd2mod, and sd2mod-licBM2, in which the wild-type and modified gene sequences were fused in frame with the reporter gene encoding thermostable lichenase, were constructed. Expression of the wild-type, modified, and hybrid genes was examined in the cells of pro- and eukaryotes. It was demonstrated that these genes were efficiently expressed in the cells of lower eukaryotes, the yeast. Inhibiting effect of the SD2 and SDmod proteins as the components of the hybrid proteins, SD2-LicBM2 and SD2mod-LicBM2, on the growth of the Fusarium culmorum hyphae was similar to that of the wild-type and modified proteins. It was shown that the presence of lichenase in the hybrid proteins facilitated selection and analysis of the hybrid proteins expression in transgenic organisms.
Russian Journal of Genetics – Springer Journals
Published: Dec 14, 2005
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