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(1989)
Chloride conductance of amphibian skin. Localization to paracellular pathways
E. Larsen, P. Kristensen (1978)
Properties of a conductive cellular chloride pathway in the skin of the toad (Bufo bufo).Acta physiologica Scandinavica, 102 1
(1990)
Do ̈rge, A
E. Larsen, B. Rasmussen (1982)
Chloride channels in toad skin.Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 299 1097
Uri Katz, C. Scheffey (1986)
The voltage-dependent chloride current conductance of toad skin is localized to mitochondria-rich cells.Biochimica et biophysica acta, 861 3
C. Scheffey (1988)
Two approaches to construction of vibrating probes for electrical current measurement in solutionReview of Scientific Instruments, 59
Daftar Pustaka, Taksonomi Organisme, berdasarkan Sejarah, Evolusi.Balai Besar, Penelitian Veteriner, Direktorat Jenderal, Gurame Penggerak, Molekuler SITH-ITB.BandungUPI (1995)
Tight junctions and the molecular basis for regulation of paracellular permeability.The American journal of physiology, 269 4 Pt 1
E. Larsen, B. Harvey (1994)
Chloride currents of single mitochondria‐rich cells of toad skin epithelium.The Journal of Physiology, 478
Jakob Sørensen, Erik Larsen (1996)
Heterogeneity of chloride channels in the apical membrane of isolated mitochondria-rich cells from toad skinThe Journal of General Physiology, 108
E. Larsen (1991)
Chloride transport by high-resistance heterocellular epithelia.Physiological reviews, 71 1
P. Kristensen (1981)
Is chloride transfer in frog skin localized to a special cell type?Acta physiologica Scandinavica, 113 1
K. Bruus, Poul Kristensen, E. Larsen (1976)
Pathways for chloride and sodium transport across toad skin.Acta physiologica Scandinavica, 97 1
P. Kristensen (1982)
CHLORIDE TRANSPORT IN FROG SKIN
(1986)
Membrane potentials and intracellular Cl activity of toad skin epithelium in relation to activation of the transepithelial Cl conductance
C. Scheffey, A. Shipley, J. Durham (1991)
Localization and regulation of acid-base secretory currents from individual epithelial cells.The American journal of physiology, 261 6 Pt 2
W. Nagel, J. García-Díaz, A. Essig (1983)
Cellular and paracellular conductance patterns in voltage-clamped frog skin.Progress in clinical and biological research, 126
The pathway for the voltage-activated chloride current across isolated toad skin was analyzed using a scanning 2D-vibrating voltage probe technique, which permits discrimination of local current peaks if their origins are more than 50 μm apart. The epithelium was separated from the corial connective tissue after enzymatic digestion with collagenase. Cl− current was activated by voltage clamping the transepithelial potential to 60–100 mV, serosa positive. Activated inward current was between 85 and 450 μA/cm2. In more than 25 tissue areas of 150 × 100 μm from 10 animals, which were automatically scanned with the vibrating probe, between 0 and 4 peaks of elevated local current (up to 800 μA/cm2) could be identified in individual fields. The density of current peaks, which were generally located at sites of mitochondria-rich (MR) cells, was less than 10% of the density of microscopically identified MR cells. The total current across individual sites of elevated conductance was 3.9 ± 0.6 nA. Considering the density of peaks, they account for 17 ± 2.5% of the applied transepithelial clamping current. The time course of current activation over previously identified conductive sites was in most cases unrelated to that of the total transepithelial current. Moreover, initially active sites could spontaneously inactivate. The results indicate that detection of elevated current above some MR cells is not sufficient to verify these cells as the pathway for transepithelial voltage-activated Cl− current. Since the major fraction of activated current is apparently not associated with a route through MR cells, channel-like structures in the tight junctions of the paracellular pathway must be considered as an alternative possibility. Current peaks over MR cells could be due to high density of such sites in tight junctions between MR and surrounding principal cells. Improvement of the spatial resolution of the vibrating probe is required to verify this view.
The Journal of Membrane Biology – Springer Journals
Published: Jan 15, 1998
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