Mitochondria as a critical element of heat shock response in yeasts with different types of energy metabolism

Mitochondria as a critical element of heat shock response in yeasts with different types of... Yeasts with different types of energy metabolism were examined with fluorescent microscopy in the presence of tetramethylrhodamine methyl ester in order to investigate the role of mitochondria in the development of a defense response to heat treatment. Heat shock was found to increase the electrochemical polarization of the inner mitochondrial membrane in an obligate aerobe Debaryomyces vanrijiae. In D. vanrijiae yeasts grown on a glucose medium, the addition of azide, 2,4-dinitrophenol, and antimycin A during “mild” heat shock treatment suppressed the development of induced thermotolerance and inhibited synthesis of a heat shock protein Hsp60. In a facultative anaerobe Saccharomyces cerevisiae grown on a glucose medium (fermentative energy metabolism), antimycin A had no effect on the development of induced thermotolerance and accumulation of a heat shock protein Hsp104. However, after growing S. cerevisiae on ethanol-containing medium under conditions of effective oxidative metabolism, antimycin A blocked the fulfillment of the defense program and prevented the enhanced synthesis of Hsp104. The results provide evidence that mitochondria play a principal role in the development of stress response and support the notion that activated transcription of stress genes depends on changes in electrochemical polarization of the inner mitochondrial membrane. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Mitochondria as a critical element of heat shock response in yeasts with different types of energy metabolism

Loading next page...
 
/lp/springer_journal/mitochondria-as-a-critical-element-of-heat-shock-response-in-yeasts-053Q2rBGAp
Publisher
Springer Journals
Copyright
Copyright © 2006 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443706050049
Publisher site
See Article on Publisher Site

Abstract

Yeasts with different types of energy metabolism were examined with fluorescent microscopy in the presence of tetramethylrhodamine methyl ester in order to investigate the role of mitochondria in the development of a defense response to heat treatment. Heat shock was found to increase the electrochemical polarization of the inner mitochondrial membrane in an obligate aerobe Debaryomyces vanrijiae. In D. vanrijiae yeasts grown on a glucose medium, the addition of azide, 2,4-dinitrophenol, and antimycin A during “mild” heat shock treatment suppressed the development of induced thermotolerance and inhibited synthesis of a heat shock protein Hsp60. In a facultative anaerobe Saccharomyces cerevisiae grown on a glucose medium (fermentative energy metabolism), antimycin A had no effect on the development of induced thermotolerance and accumulation of a heat shock protein Hsp104. However, after growing S. cerevisiae on ethanol-containing medium under conditions of effective oxidative metabolism, antimycin A blocked the fulfillment of the defense program and prevented the enhanced synthesis of Hsp104. The results provide evidence that mitochondria play a principal role in the development of stress response and support the notion that activated transcription of stress genes depends on changes in electrochemical polarization of the inner mitochondrial membrane.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Aug 29, 2006

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off