Mitigation of the liquefaction potential of soil by Ca-carbonate precipitation induced by indigenous urease-producing Staphylococcus sp. IR-103

Mitigation of the liquefaction potential of soil by Ca-carbonate precipitation induced by... Biocementation is a microbially induced technology that increases the shear strength of soil through the production of soil particle-binding materials known as calcite (calcium carbonate). This process makes use of urease-positive microorganisms, urea and calcium ions. The main aim of this study was to introduce an indigenous soil bacterium belonging to the genus Staphylococcus that was capable of hydrolyzing urea and precipitating calcium carbonate (CaCO ). Molecular identification of this isolate by 16S rDNA sequencing showed 98% homology to Staphylococcus sp. Several culture media were employed to investigate the growth, urease production and CaC O precipitation of this strain (designated Staphylococcus sp. IR-103, −1 accession number LT853888). When Staphylococcus sp. IR-103 was grown in YN medium containing 20 g L of yeast −1 extract and 10 g L of NH Cl, maximal growth yield (OD ), urease activity and carbonate precipitation values of 2.8 ± 0.1, 4 600 −1 3.33 ± 0.12 IU and 47.6 ± 0.9 mg mL were obtained, respectively. The precipitated CaC O was characterized by FTIR, AAS, XRD and SEM analyses. In order to study the effects of the bacterium’s biocementation activities on soil strength, bacterial suspension and cementation solution was injected into a column packed with uniform sandy soil under defined http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Environmental Science and Technology Springer Journals

Mitigation of the liquefaction potential of soil by Ca-carbonate precipitation induced by indigenous urease-producing Staphylococcus sp. IR-103

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Publisher
Springer Journals
Copyright
Copyright © 2018 by Islamic Azad University (IAU)
Subject
Environment; Environment, general; Environmental Science and Engineering; Environmental Chemistry; Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution; Soil Science & Conservation; Ecotoxicology
ISSN
1735-1472
eISSN
1735-2630
D.O.I.
10.1007/s13762-018-1788-6
Publisher site
See Article on Publisher Site

Abstract

Biocementation is a microbially induced technology that increases the shear strength of soil through the production of soil particle-binding materials known as calcite (calcium carbonate). This process makes use of urease-positive microorganisms, urea and calcium ions. The main aim of this study was to introduce an indigenous soil bacterium belonging to the genus Staphylococcus that was capable of hydrolyzing urea and precipitating calcium carbonate (CaCO ). Molecular identification of this isolate by 16S rDNA sequencing showed 98% homology to Staphylococcus sp. Several culture media were employed to investigate the growth, urease production and CaC O precipitation of this strain (designated Staphylococcus sp. IR-103, −1 accession number LT853888). When Staphylococcus sp. IR-103 was grown in YN medium containing 20 g L of yeast −1 extract and 10 g L of NH Cl, maximal growth yield (OD ), urease activity and carbonate precipitation values of 2.8 ± 0.1, 4 600 −1 3.33 ± 0.12 IU and 47.6 ± 0.9 mg mL were obtained, respectively. The precipitated CaC O was characterized by FTIR, AAS, XRD and SEM analyses. In order to study the effects of the bacterium’s biocementation activities on soil strength, bacterial suspension and cementation solution was injected into a column packed with uniform sandy soil under defined

Journal

International Journal of Environmental Science and TechnologySpringer Journals

Published: May 29, 2018

References

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