Microsatellites from the linkage groups E26C13 and E50C23 are located on the Gallus gallus domesticus microchromosomes 20 and 21

Microsatellites from the linkage groups E26C13 and E50C23 are located on the Gallus gallus... Using the method of dual color fluorescence in situ hybridization and a set of chromosome-specific BAC clones, localization of microsatellites LEI0345 and LEI0336 on chicken (Gallus gallus domesticus) mitotic chromosomes was performed. Microsatellite LEI0345 (TAM 32, BAC clones r49A10 and r55M23) from the linkage group E26C13 was mapped to microchromosome 20, while microsatellite LEI0336 (TAM 32, BAC clones r19E22 and r13C08) from the linkage E50C23 was assigned to microchromosome 21. Using the PCR technique, an attempt to assign the suitable markers to chromosome-specific BAC clones was made. The PCR data confirmed the microsatellite localization performed with the help of FISH technique and showed the presence of the LEI0345 microsatellite sequence on many other chicken microchromosomes, except for microchromosomes 19 and 22. Linkage groups E26C13 and E50C23 were assigned to microchromosomes 20 and 21, respectively. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Microsatellites from the linkage groups E26C13 and E50C23 are located on the Gallus gallus domesticus microchromosomes 20 and 21

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Publisher
Springer Journals
Copyright
Copyright © 2010 by Pleiades Publishing, Ltd.
Subject
Biomedicine; Microbial Genetics and Genomics; Animal Genetics and Genomics; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795410040101
Publisher site
See Article on Publisher Site

Abstract

Using the method of dual color fluorescence in situ hybridization and a set of chromosome-specific BAC clones, localization of microsatellites LEI0345 and LEI0336 on chicken (Gallus gallus domesticus) mitotic chromosomes was performed. Microsatellite LEI0345 (TAM 32, BAC clones r49A10 and r55M23) from the linkage group E26C13 was mapped to microchromosome 20, while microsatellite LEI0336 (TAM 32, BAC clones r19E22 and r13C08) from the linkage E50C23 was assigned to microchromosome 21. Using the PCR technique, an attempt to assign the suitable markers to chromosome-specific BAC clones was made. The PCR data confirmed the microsatellite localization performed with the help of FISH technique and showed the presence of the LEI0345 microsatellite sequence on many other chicken microchromosomes, except for microchromosomes 19 and 22. Linkage groups E26C13 and E50C23 were assigned to microchromosomes 20 and 21, respectively.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Apr 25, 2010

References

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