MicroRNA399 expression profiles in Arabidopsis seedlings, callus, and protoplasts in response to phosphate deficiency

MicroRNA399 expression profiles in Arabidopsis seedlings, callus, and protoplasts in response to... The functions of microRNA399 (miR399) in response to phosphate (Pi) deficiency have been extensively studied in Arabidopsis; however, previous studies have focused on relatively late responses of seedlings. In this study, the expression profiles of five miR399 primary transcripts (pri-miR399s) and mature miR399 were investigated in seedlings, calli, and mesophyll protoplasts. Pi deficiency rapidly stimulated the accumulation of pri-miR399s in the seedlings except pri-miR399b at 1 day; the amount of pri-miR399a decreased at 3 and 5 days. pri-miR399c and pri-miR399e/f showed continuously increasing patterns; the greatest accumulation of pri-miR399d was observed at 3 days. The expression of pri-miR399b, c, and f was significantly induced in the callus after 15 days of exposure to Pi deficiency. In protoplasts, the expression patterns of five pri-miR399s were comparable to those in seedlings at 1 day of Pi deficiency. Mature miR399 accumulated in the treated seedlings; and more than 460-fold of induction was observed in the calli without Pi. The expression profiles of pri-miR399s suggest that these primary transcripts are temporally and tissue-specific regulated in plant responses to Pi deficiency. Moreover, fresh isolated protoplasts could be used to study physiological perception and local signaling of Pi deficiency during early stages. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

MicroRNA399 expression profiles in Arabidopsis seedlings, callus, and protoplasts in response to phosphate deficiency

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Pleiades Publishing
Copyright © 2014 by Pleiades Publishing, Ltd.
Life Sciences; Plant Physiology; Plant Sciences
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