Review Incorporating Mouse Genome Mammalian Genome 12, 803–812 (2001). © Springer-Verlag New York Inc. 2001 DOI: 10.1007/s00335-001-4007-5 Microinsemination, nuclear transfer, and cytoplasmic transfer: the application of new reproductive engineering techniques to mouse genetics Atsuo Ogura, Narumi Ogonuki, Kaoru Takano, Kimiko Inoue Department of Veterinary Science, National Institute of Infectious Diseases, 1-23-1, Toyama, Shinjuku, Tokyo 162-8640, Japan Received: 30 June 2001 / Accepted: 16 July 2001 Mice are the most commonly used laboratory animals, owing to Microinsemination the availability of large amounts of information on their genetics and biology. As a result, numerous advanced techniques have been The development of intracytoplasmic sperm injection (ICSI) in the developed by using mouse embryos. Studies on murine develop- mouse. The first attempt to fertilize mammalian oocytes by mi- mental and reproductive biology are greatly facilitated by short croinsemination was made by Uehara and Yanagimachi in 1976. gestation and generation periods of 20 days and approximately 3 They demonstrated that an injected sperm head of the golden ham- months, respectively. Transgenic and knockout mice are of fun- ster developed into the male pronucleus after incorporation into the damental relevance to mammalian transgenic research. However, homologous ooplasm. Microinsemination of mice was first estab- in certain
Mammalian Genome – Springer Journals
Published: Apr 20, 2014
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