Access the full text.
Sign up today, get DeepDyve free for 14 days.
S. Zappe, M. Fish, M.P. Scott, O. Solgaard (2006)
Automated MEMS-based Drosophila embryo injection system for high-throughput RNAi screensLab Chip, 6
B. Houchmandzadeh, E. Wieschaus, S. Leibler (2002)
Establishment of developmental precision and proportions in the early Drosophila embryoNature, 415
X.J. Zhang, M.P. Scott, C.F. Quate, O. Solgaard (2006)
Microoptical characterization of piezoelectric vibratory microinjections in Drosophila embryos for genome-wide RNAi screenJ. Microelectromech. Syst., 15
F. He, C. Wei, H. Wu, D. Cheung, R. Jiao, J. Ma (2015)
Fundamental origins and limits for scaling a maternal morphogen gradientNat. Commun., 6
G.T. Reeves, N. Trisnadi, T.V. Truong, M. Nahmad, S. Katz, A. Stathopoulos (2012)
Dorsal-ventral gene expression in the Drosophila embryo reflects the dynamics and precision of the dorsal nuclear gradientDev. Cell, 22
G.B. Salieb-Beugelaar, G. Simone, A. Arora, A. Philippi, A. Manz (2010)
Latest developments in microfluidic cell biology and analysis systemsAnal. Chem., 82
G.T. Dagani, K. Monzo, J.R. Fakhoury, C.C. Chen, J.C. Sisson, X.J. Zhang (2007)
Microfluidic self-assembly of live Drosophila embryos for versatile high throughput analysis of embryonic morphogenesisBiomed. Microdevices, 9
E.M. Lucchetta, M.S. Munson, R.F. Ismagilov (2006)
Characterization of the local temperature in space and time around a developing Drosophila embryo in a microfluidic deviceLab Chip, 6
T.M. Zimina (2009)
Miniature analytical systems for biomedical applications—labs on chipsBiotekhnosfera, 1
R.W. Bernstein (2004)
67Proc. SPIE—Int. Soc. Opt. Engin., 5641
J.A. Campos-Ortega, V. Hartenstein (1985)
The Embryonic Development of Drosophila melanogaster
I.V. Kukhtevich, K.I. Belousov, A.S. Bukatin, A.A. Evstrapov (2015)
Topologies of microfluidic devices to study the migration of cells in gradients of chemical substances (review)Nauchn. Priborostr., 25
T.J. Levario, M. Zhan, B. Lim, S.Y. Shvartsman, H. Lu (2013)
Microfluidic trap array for massively parallel imaging of Drosophila embryosNat. Protoc., 8
M.M. Witzberger, J.A.J. Fitzpatrick, J.C. Crowley, J.S. Minden (2008)
End-on imaging: a new perspective on dorsoventral development in Drosophila embryosDev. Dyn., 237
T. Gregor, E.F. Wieschaus, A.P. McGregor, W. Bialek, D.W. Tank (2007)
Stability and nuclear dynamics of the Bicoid morphogen gradientCell, 130
D. Delubac, C.B. Highley, M. Witzberger-Krajcovic, J.C. Ayoob, E.C. Furbee (2012)
Microfluidic system with integrated microinjector for automated Drosophila embryo injectionLab Chip, 12
X.J. Zhang, C.-C. Chen, R.W. Bernstein, S. Zappe, M.P. Scott, O. Solgaard (2005)
Microoptical characterization and modeling of positioning forces on Drosophila embryos self-assembled in two-dimensional arraysJ. Microelectromech. Syst., 14
S. Bergmann, O. Sandler, H. Sberro, S. Shnider, E. Schejter, B.-Z. Shilo, N. Barkai (2007)
Pre-steady-state decoding of the Bicoid morphogen gradientPLoS Biol., 5
L.A. Royer, W.C. Lemon, R.K. Chhetri, Y. Wan, M. Coleman, E.W. Myers, P.J. Keller (2016)
Adaptive lightsheet microscopy for long-term, high-resolution imaging in living organismsNat. Biotechnol., 34
S.Ki.m Kim, N.L. Jeon (2010)
Biological applications of microfluidic gradient devicesIntegr. Biol., 2
E.M. Lucchetta, R.W. Carthew, R.F. Ismagilov (2009)
The Endo-siRNA pathway is essential for robust development of the Drosophila embryoPLoS One, 4
A.C. Spradling, D. Stern, A. Beaton, E.J. Rhem, T. Laverty (1999)
The Berkeley Drosophila Genome Project gene disruption project: single P-element insertions mutating 25% of vital Drosophila genesGenetics, 153
C.C. Chen, S. Zappe, O. Sahin, X.J. Zhang, M. Fish, M. Scott, O. Solgaard (2004)
Design and operation of a microfluidic sorter for Drosophila embryosSens. Actuators B: Chem., 102
T.J. Levario, C. Zhao, T. Rouse, S.Y. Shvartsman, H. Lu (2016)
An integrated platform for large-scale data collection and precise perturbation of live Drosophila embryosSci. Rep., 6
E.E.M. Furlong, D. Profitt, M.P. Scott (2001)
Automated sorting of live transgenic embryosNat. Biotechnol., 19
I.S. Peter, E.H. Davidson (2013)
Handbook of Systems Biology. Concepts and Insights
A.D. Meer, A.A. Poot, M.H.G. Duits, J. Feijen, I. Vermes (2009)
J. Biomed. Biotechnol.
A. v d Raj, P. Bogaard, S.A. v Rifkin, A. Oudenaarden, S. Tyagi (2008)
Imaging individual mRNA molecules using multiple singly labeled probesNat. Methods, 5
M.L. Zanaveskin, A.A. Mironova, A.M. Popov (2012)
Microfluidics and its prospects in medicineMol. Med.: Kvart. Nauch.-Prakt. Zh., 5
D.J. Beebe (2002)
261Annu. Rev. Biomed. Eng., 4
W. Busch, B.T. Moore, B. Martsberger, D.L. Mace, R.W. Twigg (2012)
A microfluidic device and computational platform for high-throughput live imaging of gene expressionNat. Methods, 9
O. Crauk, N. Dostatni (2005)
Bicoid determines sharp and precise target gene expression in the Drosophila embryoCurr. Biol., 15
X.J. Feng, W. Du, Q.M. Luo, B.F. Liu (2009)
Microfluidic chip: next-generation platform for systems biologyAnal. Chim. Acta, 650
K. Chung, Y. Kim, J.S. Kanodia, E. Gong, S.Y. Shvartsman, H. Lu (2011)
A microfluidic array for large-scale ordering and orientation of embryosNat. Methods, 8
Y. Zhang, L.C. Yu (2008)
Single-cell microinjection technology in cell biologyBioEssays, 30
R.W. Bernstein (2004)
191Sensors Actuators A: Physical, 114
M.D. Adams, S.E. Celniker, R.A. Holt, C.A. Evans, J.D. Gocayne (2000)
The genome sequence of Drosophila melanogasterScience, 287
M.D. Adams (2000)
2185Science, 287
J.Y. Wang, L. Ren, L. Li, W.M. Liu, J. Zhou, W.H. Yu, D.W. Tong, S.L. Chen (2009)
Microfluidics: a new cosset for neurobiologyLab Chip, 9
D.M. Holloway, L.G. Harrison, D. Kosman, C.E. Vanario-Alonso, A.V. Spirov (2006)
Analysis of pattern precision shows that Drosophila segmentation develops substantial independence from gradients of maternal gene productsDev. Dyn., 235
T.J. Levario, B. Lim, S.Y. Shvartsman, H. Lu (2016)
Microfluidics for high-throughput quantitative studies of early developmentAnnu. Rev. Biomed. Eng., 18
R.W. Bernstein, X. Zhang, S. Zappe, M. Fish, M.P. Scott, O. Solgaard (2004)
Characterization of fluidic microassembly for immobilization and positioning of Drosophila embryos in 2-D arraysSensors Actuators A: Physical, 114
I.V. Kukhtevich, A.A. Evstrapov, A.S. Bukatin (2013)
Microfluidic device for cell studiesNauchn. Priborostr., 23
S. Bergmann (2007)
232PLoS Biol., 5
J. El-Ali, P.K. Sorger, K.F. Jensen (2006)
Nature
A. Webster, J. Greenman, S.J. Haswell (2011)
Development of microfluidic devices for biomedical and clinical applicationJ. Chem. Technol. Biotechnol., 86
E.M. Lucchetta, J.H. Lee, L.A. Fu, N.H. Patel, R.F. Ismagilov (2005)
Dynamics of Drosophila embryonic patterning network perturbed in space and time using microfluidicsNature, 434
J. Kanodia, H.-L. Liang, Y. Kim, B. Lim, M. Zhan, H. Lu, C. Rushlow, S. Shvartsman (2012)
Pattern formation by graded and uniform signals in the early Drosophila embryoBiophys. J., 102
R. McGorty, H. Liu, D. Kamiyama, Z.Q. Dong, S. Guo, B. Huang (2015)
Open-top selective plane illumination microscope for conventionally mounted specimensOpt. Express., 23
H. Bruus (2006)
Theoretical Microfluidics. Lecture Notes
J.R. Fakhoury, J.C. Sisson, X.J. Zhang (2009)
Microsystems for controlled genetic perturbation of live Drosophila embryos: RNA interference, development robustness and drug screeningMicrofluid. Nanofluid., 6
D.J. Beebe, G.A. Mensing, G.M. Walker (2002)
Physics and applications of microfluidics in biologyAnnu. Rev. Biomed. Eng., 4
T.C. v Esteves, F. Rossem, V. Nordhoff, S. Schlatt, M. L. Boiani, S. Gac (2013)
A microfluidic system supports single mouse embryo culture leading to full-term developmentR. Soc. Chem. Adv., 3
A.A. Evstrapov (2011)
Microfluidic chips for biological and medical researchRoss. Khim. Zh., 55
E.K. Kieserman, M. Glotzer, J.B. Wallingford (2008)
Developmental regulation of central spindle assembly and cytokinesis during vertebrate embryogenesisCurr. Biol., 18
F. He, Y. Wen, J. Deng, X. Lin, L.J. Lu, R. Jiao, J. Ma (2008)
Probing intrinsic properties of a robust morphogen gradient in DrosophilaDev. Cell, 15
K. Ziolkowska, R. Kwapiszewski, Z. Brzozka (2011)
Microfluidic devices as tools for mimicking the in vivo environmentNew J. Chem., 35
A.V. Spirov, D.M. Holloway (2003)
Making the body plan: precision in the genetic hierarchy of Drosophila embryo segmentationIn Silico Biol., 3
G.M. Whitesides, E. Ostuni, S. Takayama (2001)
Soft lithography in biology and biochemistryAnnu. Rev. Biomed. Eng., 3
A.A. Evstrapov (2011)
Nanosize structures in microfluidic devices (review)Nauchn. Priborostr., 21
E.M. Lucchetta, M.E. Vincent, R.F. Ismagilov (2008)
A precise Bicoid gradient is nonessential during cycles 11–13 for precise patterning in the Drosophila blastodermPLoS One, 3
J. Krajniak, H. Lu (2010)
Long-term high-resolution imaging and culture of C. elegans in chip-gel hybrid microfluidic device for developmental studiesLab Chip, 10
J.S. Kanodia, Y. Kim, R. Tomer, Z. Khan, K. Chung, J.D. Storey, H. Lu, P.J. Keller, S.Y. Shvartsman (2011)
A computational statistics approach for estimating the spatial range of morphogen gradientsDevelopment, 138
T. Squires, S. Quake (2005)
Microfluidics: fluid physics at the nanoliter scaleRevs. Mod. Phys., 77
M.D. Petkova, S.C. Little, F. Liu, T. Gregor (2014)
Maternal origins of developmental reproducibilityCurr. Biol., 24
S.C. Little, M. Tikhonov, T. Gregor (2013)
Precise developmental gene expression arises from globally stochastic transcriptional activityCell, 154
R.W. Bernstein, M. Scott, O. Solgaard (2004)
BioMEMS for high-throughput handling and microinjection of embryosProc. SPIE—Int. Soc. Opt. Engin., 5641
D. v Choudhury, D. Noort, C. Iliescu, B.X. Zheng, K.L. Poon (2012)
Fish and chips: a microfluidic perfusion platform for monitoring zebrafish developmentLab Chip, 12
A.M. Taylor, N.L. Jeon (2010)
Micro-scale and microfluidic devices for neurobiologyCurr. Opin. Neurobiol., 20
Modern automated microsystems based on microhydrodynamic (microfluidic) technologies— labs on chips—make it possible to solve various basic and applied research problems. In the last 15 years, the development of these approaches in application to the problems of modern quantitative (systems) development biology has been observed. In this field, high-throughput experiments aimed at accumulating ample quantitative data for their subsequent computer analysis are important. In this review, the main directions in the development and application of microfluidics approaches for solving problems of modern developmental biology using the classical model object, Drosophila embryo, as an example is discussed. Microfluidic systems provide an opportunity to perform experiments that can hardly be performed using other approaches. These systems allow automated, rapid, reliable, and proper placing of many live embryos on a substrate for their simultaneous confocal scanning, sorting them, or injecting them with various agents. Such systems make it possible, in particular, to create controlled gradients of microenvironmental parameters along a series of developing embryos or even to introduce discontinuity in parameters within the microenvironment of one embryo, so that the head half is under other conditions compared to the tail half (at continuous scanning). These approaches are used both in basic research of the functions of gene ensembles that control early development, including the problems of resistance of early patterns to disturbances, and in test systems for screening chemical agents on developing embryos. The problems of integration of microfluidic devices in systems for automated performance of experiments simultaneously on many developing embryos under conditions of their continuous scanning using modern fluorescence microscopy instruments will be discussed. The methods and approaches developed for Drosophila are also applicable to other model objects, even mammalian embryos.
Russian Journal of Developmental Biology – Springer Journals
Published: Jun 1, 2018
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.