Microarray analysis of differentially expressed transcripts in porcine intestinal epithelial cells (IPEC-J2) infected with porcine sapelovirus as a model to study innate immune responses to enteric viruses

Microarray analysis of differentially expressed transcripts in porcine intestinal epithelial... The local intestinal mucosa, the largest mucosal immune system in animals, plays an important role in resistance against intestinal pathogen infection. However, the molecular antiviral mechanisms of the intestinal mucosa remain poorly understood. In this study, we screened and identified differentially expressed transcripts in (PSV) porcine intestinal epithelial cells (IPEC-J2) infected with porcine sapelovirus using microarray analysis. A total of 2298 differentially expressed genes were screened at four time points during PSV infection. These genes were involved in numerous physical systems and molecular pathways, and particularly, some innate immune-associated pathways were significant. The results showed that large amounts of type I interferon were induced, and the related interferon effect pathway was activated when IPEC-J2 cells were infected with PSV. Three pathways of innate immune receptors, including Toll-like, NOD-like, and RIG-I-like receptors, were also activated. The antigen was then processed and presented through the MHCI and MHCII pathways. Interestingly, we found that the secretion network of IgA was activated in the early stage of PSV infection. Two exogenous and endogenous apoptosis pathways were also activated during PSV infection. The results revealed changes in gene transcription, particularly those of innate immune pathway genes that were associated with PSV infection in IPEC-J2 cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Microarray analysis of differentially expressed transcripts in porcine intestinal epithelial cells (IPEC-J2) infected with porcine sapelovirus as a model to study innate immune responses to enteric viruses

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Publisher
Springer Vienna
Copyright
Copyright © 2013 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1638-2
Publisher site
See Article on Publisher Site

Abstract

The local intestinal mucosa, the largest mucosal immune system in animals, plays an important role in resistance against intestinal pathogen infection. However, the molecular antiviral mechanisms of the intestinal mucosa remain poorly understood. In this study, we screened and identified differentially expressed transcripts in (PSV) porcine intestinal epithelial cells (IPEC-J2) infected with porcine sapelovirus using microarray analysis. A total of 2298 differentially expressed genes were screened at four time points during PSV infection. These genes were involved in numerous physical systems and molecular pathways, and particularly, some innate immune-associated pathways were significant. The results showed that large amounts of type I interferon were induced, and the related interferon effect pathway was activated when IPEC-J2 cells were infected with PSV. Three pathways of innate immune receptors, including Toll-like, NOD-like, and RIG-I-like receptors, were also activated. The antigen was then processed and presented through the MHCI and MHCII pathways. Interestingly, we found that the secretion network of IgA was activated in the early stage of PSV infection. Two exogenous and endogenous apoptosis pathways were also activated during PSV infection. The results revealed changes in gene transcription, particularly those of innate immune pathway genes that were associated with PSV infection in IPEC-J2 cells.

Journal

Archives of VirologySpringer Journals

Published: Jul 1, 2013

References

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