Plant Mol Biol (2011) 77:533–536 DOI 10.1007/s11103-011-9834-5 RESPONSE TO LETT ER TO THE E DI TOR M. I. Vaquero-Sedas and M. A. Vega-Palas: DNA methylation at tobacco telomeric sequences • • ´ ´ Eva Majerova Miloslava Fojtova Terezie Manda ´ kova ´ Jir ˇ´ ı Fajkus Received: 20 September 2011 / Accepted: 6 October 2011 / Published online: 4 November 2011 Springer Science+Business Media B.V. 2011 Recently, we reported (Majerova et al. 2011) that hy- repeats) form 6.3 kb distributed into several blocks of pomethylating drugs efﬁciently decrease methylation of 300–1,200 bp and exhibit a various degree of sequence telomeric DNA and activate telomerase without affecting degeneracy (Uchida et al. 2002). These are termed more telomere lengths in tobacco. The key issue of the Letter to appropriately as interstitial telomeric repeats (ITRs) and Editor written by Vaquero-Sedas and Vega-Palas (2011) is, they are presumably heterochromatic, as would correspond whether our results demonstrating methylation and drug- to their subtelomeric or pericentromeric location. They can induced hypomethylation of tobacco telomeric DNA be detected by Southern hybridization (during terminal repeats do correspond to the status of terminal telomeric restriction fragment analyses) as a signal of about 0.5 kb sequences, or rather to
Plant Molecular Biology – Springer Journals
Published: Nov 4, 2011
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