Methods for array tomography with correlative light and electron microscopy

Methods for array tomography with correlative light and electron microscopy The three-dimensional ultra-structure is the comprehensive structure that cannot be observed from a two-dimensional electron micrograph. Array tomography is one method for three-dimensional electron microscopy. In this method, to obtain consecu- tive cross sections of tissue, connected consecutive sections of a resin block are mounted on a flat substrate, and these are observed with scanning electron microscopy. Although array tomography requires some bothersome manual procedures to prepare specimens, a recent study has introduced some techniques to ease specimen preparation. In addition, array tomog- raphy has some advantages compared with other three-dimensional electron microscopy techniques. For example, sections on the substrate are stored semi-eternally, so they can be observed at different magnifications. Furthermore, various stain- ing methods, including post-embedding immunocytochemistry, can be adopted. In the present review, the preparation of specimens for array tomography, including ribbon collection and the staining method, and the adaptability for correlative light and electron microscopy are discussed. Keywords Three-dimensional electron microscopy · Array tomography · Correlative light and electron microscopy · Pre- embedding staining · Post-embedding staining Introduction (SBF/SEM) or focused ion beam SEM (FIB/SEM), respec- tively [2, 3]. The three-dimensional ultra-structure has been determined Array tomography is an imaging technique that was pro- with transmission electron microscopy (TEM) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Medical Molecular Morphology Springer Journals

Methods for array tomography with correlative light and electron microscopy

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Publisher
Springer Journals
Copyright
Copyright © 2018 by The Japanese Society for Clinical Molecular Morphology
Subject
Medicine & Public Health; Pathology; Anatomy; Molecular Medicine
ISSN
1860-1480
eISSN
1860-1499
D.O.I.
10.1007/s00795-018-0194-y
Publisher site
See Article on Publisher Site

Abstract

The three-dimensional ultra-structure is the comprehensive structure that cannot be observed from a two-dimensional electron micrograph. Array tomography is one method for three-dimensional electron microscopy. In this method, to obtain consecu- tive cross sections of tissue, connected consecutive sections of a resin block are mounted on a flat substrate, and these are observed with scanning electron microscopy. Although array tomography requires some bothersome manual procedures to prepare specimens, a recent study has introduced some techniques to ease specimen preparation. In addition, array tomog- raphy has some advantages compared with other three-dimensional electron microscopy techniques. For example, sections on the substrate are stored semi-eternally, so they can be observed at different magnifications. Furthermore, various stain- ing methods, including post-embedding immunocytochemistry, can be adopted. In the present review, the preparation of specimens for array tomography, including ribbon collection and the staining method, and the adaptability for correlative light and electron microscopy are discussed. Keywords Three-dimensional electron microscopy · Array tomography · Correlative light and electron microscopy · Pre- embedding staining · Post-embedding staining Introduction (SBF/SEM) or focused ion beam SEM (FIB/SEM), respec- tively [2, 3]. The three-dimensional ultra-structure has been determined Array tomography is an imaging technique that was pro- with transmission electron microscopy (TEM)

Journal

Medical Molecular Morphology – Springer Journals

Published: May 31, 2018

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