The chemical thermodynamic concept for membrane electroporation is critically revisited. The hysteresis in the electric field dependence of the rapid in-field electroporation events (on the in-field hysteresis branch) and the slower post-field pore resealing process (zero-field hysteresis branch) is a typical ensemble property involving rapid single-pore opening–closing events that are temporally and spatially distributed. In the case of spherical membrane shells in homogeneous external fields, the acting local field is dependent on the polar–angular position. Hence, the experimental state distribution constant and the ensemble rate coefficients are statistical position averages; they are cosine square averages of the polar angle. Advanced flux analysis uses the concept of time-dependent flux coefficients reflecting the kinetics of the rate-limiting structural processes of electroporation and membrane resealing. The explicit integral flux equations rationalize the sigmoid onset of the in-field kinetics and quantify the post-field-stretched exponentials as exponentials of exponentials. Finally, the new analytical proposal for the evaluation of the electric field strength dependence of global cell electroporation data starts with the low-field range and continues with iterative parameter optimisation over the entire field strength range.
European Biophysics Journal – Springer Journals
Published: May 8, 2018
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
All the latest content is available, no embargo periods.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud