Membrane electroporation: chemical thermodynamics and flux kinetics revisited and refined

Membrane electroporation: chemical thermodynamics and flux kinetics revisited and refined The chemical thermodynamic concept for membrane electroporation is critically revisited. The hysteresis in the electric field dependence of the rapid in-field electroporation events (on the in-field hysteresis branch) and the slower post-field pore resealing process (zero-field hysteresis branch) is a typical ensemble property involving rapid single-pore opening–closing events that are temporally and spatially distributed. In the case of spherical membrane shells in homogeneous external fields, the acting local field is dependent on the polar–angular position. Hence, the experimental state distribution constant and the ensemble rate coefficients are statistical position averages; they are cosine square averages of the polar angle. Advanced flux analysis uses the concept of time-dependent flux coefficients reflecting the kinetics of the rate-limiting structural processes of electroporation and membrane resealing. The explicit integral flux equations rationalize the sigmoid onset of the in-field kinetics and quantify the post-field-stretched exponentials as exponentials of exponentials. Finally, the new analytical proposal for the evaluation of the electric field strength dependence of global cell electroporation data starts with the low-field range and continues with iterative parameter optimisation over the entire field strength range. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Biophysics Journal Springer Journals

Membrane electroporation: chemical thermodynamics and flux kinetics revisited and refined

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Publisher
Springer International Publishing
Copyright
Copyright © 2018 by European Biophysical Societies' Association
Subject
Life Sciences; Biochemistry, general; Biological and Medical Physics, Biophysics; Cell Biology; Neurobiology; Membrane Biology; Nanotechnology
ISSN
0175-7571
eISSN
1432-1017
D.O.I.
10.1007/s00249-018-1305-3
Publisher site
See Article on Publisher Site

Abstract

The chemical thermodynamic concept for membrane electroporation is critically revisited. The hysteresis in the electric field dependence of the rapid in-field electroporation events (on the in-field hysteresis branch) and the slower post-field pore resealing process (zero-field hysteresis branch) is a typical ensemble property involving rapid single-pore opening–closing events that are temporally and spatially distributed. In the case of spherical membrane shells in homogeneous external fields, the acting local field is dependent on the polar–angular position. Hence, the experimental state distribution constant and the ensemble rate coefficients are statistical position averages; they are cosine square averages of the polar angle. Advanced flux analysis uses the concept of time-dependent flux coefficients reflecting the kinetics of the rate-limiting structural processes of electroporation and membrane resealing. The explicit integral flux equations rationalize the sigmoid onset of the in-field kinetics and quantify the post-field-stretched exponentials as exponentials of exponentials. Finally, the new analytical proposal for the evaluation of the electric field strength dependence of global cell electroporation data starts with the low-field range and continues with iterative parameter optimisation over the entire field strength range.

Journal

European Biophysics JournalSpringer Journals

Published: May 8, 2018

References

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